CoChat
Sign Up
Cited by 33Olfactory and Sensory Function StudiesAnimal Virus Infections StudiesNeurobiology and Insect Physiology Research

Functional Consequences Following Infection of the Olfactory System by Intranasal Infusion of the Olfactory Bulb Line Varient (OBLV) of Mouse Hepatitis Strain JHM

Open access
Youngentob, S. L., Schwob, J. E., Saha, S., Manglapus, G., Jubelt, B.·2001·Chemical Senses
DOI 10.1093/chemse/26.8.953PMC PMC7110209
Open this paper in CoChat — free account

Abstract

The present study assessed the functional consequences of viral infection with a neurotropic coronavirus, designated MHV OBLV, that specifically targets central olfactory structures. Using standard operant techniques and a 'go, no-go' successive discrimination paradigm, six BALB/c mice were trained to discriminate between the presentation of an air or odor stimulus (three mice for each of the odorants propanol and propyl acetate). Two additional BALB/c mice were trained to discriminate between the presentation of air and the presentation of either vanillin or propionic acid. Following criterion performance, each mouse received an additional 2000 trials of overtraining. At completion of overtraining one mouse from the propanol and propyl acetate groups were allocated as untreated. The remaining six mice were inoculated with 300 microl of the OBLV stock per nostril for a total of 1.5 x 10(6) p.f.u. in 600 microl. Following a 1 month rest, untreated and inoculated animals were again tested on their respective air versus odor discrimination task. Untreated animals immediately performed at criterion levels. In contrast, inoculated animals varied in their capacity to discriminate between air and odorant. Five of the six inoculated mice showed massive disruption of the olfactory bulb, including death of mitral cells; the other was more modestly affected. In addition, the density of innervation of the olfactory mucosa by substance P-containing trigeminal fibers is also affected by inoculation. Those mice that remained anosmic to the training odorants had the most severe reduction in mitral cell number and substance P fiber density among the inoculated animals.

Integrity signals

Registry

Crossref · 10.1093/chemse/26.8.953

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Rodent coronavirus MHV passes from nose to bulb even though rodent olfactory sensory neurons do not express Ceacam1, the main MHV receptor.

    “The rodent coronavirus MHV passes from the nose to the bulb, even though rodent OSNs do not express Ceacam1, the main MHV receptor (61, 66) (Figures S4C, S5E, S6A), suggesting that CoVs in the nasal mucosa can reach the brain through mechanisms independent of axonal transport by sensory nerves; interestingly, OB dopaminergic juxtaglomerular cells express Ceacam1 (Fig. 7E), which likely supports”

    From Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia (2020) · cites this paper as [61]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Youngentob, S. L., Schwob, J. E., Saha, S., Manglapus, G., & Jubelt, B.
Affiliations
  • SUNY Upstate Medical University (US)
  • Tufts University (US)
Corresponding author
Youngentob, S. L.
ORCID
Schwob, J. E.
Venue
Chemical Senses
Publisher
Oxford University Press (OUP)
Published
2001-10-01
Volume
26
Issue
8
Pages
953-963
ISSN
  • 0379-864X (ISSN-L)
  • 1464-3553
DOI
10.1093/chemse/26.8.953
PMC
PMC7110209
OpenAlex
W2130961194
Cited by
33
References
42
Citations by year
2025: 1 · 2024: 2 · 2023: 2 · 2021: 4 · 2020: 10 · 2018: 1 · 2017: 1 · 2014: 1 · 2012: 2
FWCI
0.47
Type
journal article
Language
en
Open access
Locations
  • Chemical Senses (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01dc003904-05)
  • National Institute on Deafness and Other Communication Disorders (P01DC000220, P01-DC00220, R01 DC03904)
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
neurotropic coronavirus, olfactory bulb, olfactory dysfunction, anosmia, odor discrimination, substance P, viral encephalitis
MeSH terms
Administration, Intranasal, Air, Animals, Benzaldehydes, Murine hepatitis virus, Male, Mice, Inbred BALB C, Olfactory Bulb, Propionates, Smell, Substance P, Time Factors, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review