Preparative Scale Purification of Shidasterone, 2-Deoxy-polypodine B and 9 , 20-Dihydroxyecdysone from Silene italica ssp. nemoralis
Abstract
A suitable combination of preparative scale separation methods results in effective clean-up of the ecdysteroids of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The isolation of minor ecdysteroids from the partially purified extract is based on the use of both droplet counter-current chromatography and low-pressure reversed-phase liquid chromatography. The purification is completed by preparative thin-layer chromatography and preparative high-performance liquid chromatography to obtain the minor ecdysteroids, such as 2-deoxy-20-hydroxyecdysone, shidasterone, 2-deoxy-polypodine B, makisterone C, and 9alpha,20-dihydroxyecdysone.
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DCCC is an effective technique for the purification of ecdysteroids of wide polarity range from complex mixtures.
“DCCC, which is a preparative partition system between immiscible stationary and mobile phases, is an effective technique for the purification of ecdysteroids of wide polarity range from complex mixtures [119-121] since it is non-destructive and all components elute at some point during the chromatography”
From Ecdysteroids (2021) · cites this paper as [121]
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Sticher, O. (2008) · Natural Product Reports · doi:10.1039/b700306b · Cited by 378 · see citing works
Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
Huang, J., Xu, X., Xie, Z., Xie, Z., Yang, M., & Yang, D. (2014) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826076.2013.825850 · Cited by 4 · see citing works
“In the past, the methods of purifying 20-Hydroxyecdysone from Radix Serratulae Chinensis were column chromatography and droplet counter-current chromatography,([6,7]) which usually required multiple complicated steps and was time consuming.”
MethodECDYSTEROIDS OF SILENE ITALICA: GLYCOSIDIC AND NONGLYCOSIDIC COMPONENTS AND HPLC-DAD-ESI-MS PROFILE
Olennikov, D. N., & Kashchenko, N. I. (2019) · chemistry of plant raw material · doi:10.14258/jcprm.2019045109 · Cited by 4 · see citing works
Current Awareness in Phytochemical Analysis
(2004) · Phytochemical Analysis · doi:10.1002/pca.751
Silene genus: a review of its traditional uses, phytochemistry and pharmacology
BILAL, M. N., & Kutluay, V. M. (2025) · Phytochemistry Reviews · doi:10.1007/s11101-025-10208-4 · Cited by 4 · see citing works
- Authors
- Bathori, M., Pongracz, Z., Omacht, R., & Mathe, I.
- Affiliations
- University of Pecs (HU)
- Venue
- Journal of Chromatographic Science
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-05-01
- Volume
- 42
- Issue
- 5
- Pages
- 275-279
- ISSN
- 0021-9665 (ISSN-L)
- 1945-239X
- OpenAlex
- W2325030863
- Cited by
- 6
- References
- 0
- Citations by year
- 2025: 1 · 2021: 1 · 2019: 1 · 2013: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatographic Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- ecdysteroids, reversed-phase liquid chromatography, preparative thin-layer chromatography, preparative HPLC
- MeSH terms
- Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Countercurrent Distribution, Ecdysone, Structure-Activity Relationship, Ecdysteroids, Silene
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