ISOLATION AND PURIFICATION OF 20-HYDROXYECDYSONE FROM RADIX SERRATULAE CHINENSIS BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
Abstract
□ The bioactive compound 20-Hydroxyecdysone was successfully isolated and purified from Radix Serratulae Chinensis by high-speed counter-current chromatography with a solvent system of ethyl acetate–n-butanol–water (4:1:5, v/v/v) in one step. From 230mg of the n-butanol extract of Radix Serratulae Chinensis, 16.7mg of 20-Hydroxyecdysone was obtained with purity of 99.3%, as determined by high performance liquid Chromatography (HPLC). The structure was identified by mass spectrophotometry (MS), ultraviolet spectrophotometry (UV), and nuclear magnetic resonance spectrophotometry (NMR) analysis. In this study, a rapid method for isolation and purification of the major compound from Radix Serratulae Chinensis extract was established.
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Only 20-hydroxyecdysone was successfully isolated by HSCCC from Radix Serratulae Chinensis.
“Only 20-hydroxyecdysone was successfully isolated by means of HSCCC from Radix Serratulae Chinensis [14].”
Cites
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“In the past, the methods of purifying 20-Hydroxyecdysone from Radix Serratulae Chinensis were column chromatography and droplet counter-current chromatography,([6,7]) which usually required multiple complicated steps and was time consuming.”
MethodECDYSTEROIDS FROM THE STEMS OF DIPLOCLISIA GLAUCESCENS
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Ecdysteroids from the Roots of Serratula chinensis
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Fang, L., Li, J., Zhou, J., Wang, X., & Guo, L. (2017) · Molecules · doi:10.3390/molecules22081310 · Cited by 20 · see citing works
Issaadi, H. M., Tsai, Y.-C., Chang, F.-R., & Hunyadi, A. (2017) · Journal of Chromatography B · doi:10.1016/j.jchromb.2017.03.043 · Cited by 19 · see citing works
- Authors
- Huang, J., Xu, X., Xie, Z., Xie, Z., Yang, M., & Yang, D.
- Affiliations
- Sun Yat-sen University (CN)
- a School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou , P. R. China
- Corresponding author
- Xu, Xinjun
- Venue
- Journal of Liquid Chromatography & Related Technologies
- Publisher
- Informa UK Limited
- Published
- 2014-04
- Volume
- 37
- Issue
- 13
- Pages
- 1909-1916
- ISSN
- 1082-6076 (ISSN-L)
- 1520-572X
- OpenAlex
- W1967697447
- Cited by
- 4
- References
- 9
- Citations by year
- 2018: 1 · 2017: 2 · 2016: 1
- FWCI
- 0.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Liquid Chromatography & Related Technologies (publishedVersion)
- Funders
- This work was supported by the International Scientific and Technological Cooperation Program of China (No: 2009DFA31230) and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No: 2010B090400533).
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- 20-hydroxyecdysone, high-speed countercurrent chromatography, steroid isolation, structural identification
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