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Direct Spectrophotometric Determination of Albumin in Human Serum
Abstract
In a convenient and direct method for determination of albumin in human serum, based on the decrease in absorbance of a neutral buffered solution of bromcresol green when albumin combines with the indicator, serum is diluted with a solution of bromcresol green of sufficient concentration to allow essentially linear change of absorbance with albumin concentration. Measurements are made at 615 mµ, where the absorbance of hemoglobin or bilirubin does not interfere. Extremely lipemic, turbid serums may be accurately analyzed. This method has been applied to a number of abnormal serums and compared with measurements of the albumin fraction of serum which is soluble in 2% (w/v) TCA in ethanol. Satisfactory agreement was obtained. Tests indicated that bromcresol green was not bound by serum globulins.
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Cited by
Albumin standards and the measurement of serum albumin with bromcresol green
Doumas, B. T., Ard Watson, W., & Biggs, H. G. (1997) · Clinica Chimica Acta · doi:10.1016/s0009-8981(96)06447-9 · Cited by 2,767
Södergard, R., Bäckström, T., Shanbhag, V., & Carstensen, H. (1982) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(82)90038-3 · Cited by 1,080
Protein losses during peritoneal dialysis
Blumenkrantz, M. J., Gahl, G. M., Kopple, J. D., Kamdar, A. V., Jones, M. R., Kessel, M., & Coburn, J. W. (1981) · Kidney International · doi:10.1038/ki.1981.57 · Cited by 380
Albumin homeostasis in patients undergoing continuous ambulatory peritoneal dialysis
Kaysen, G. A., & Schoenfeld, P. Y. (1984) · Kidney International · doi:10.1038/ki.1984.15 · Cited by 193
HAMMOND, G. L., LEINONEN, P., BOLTON, N. J., & VIHKO, R. (1983) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1983.tb00582.x · Cited by 17
Metadata
- Authors
- Rodkey, F. L.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1965-04-01
- Volume
- 11
- Issue
- 4
- Pages
- 478-487
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1922526566
- Cited by
- 415
- References
- 0
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rodkey, F. L. (1965). Direct Spectrophotometric Determination of Albumin in Human Serum. Clinical Chemistry. https://doi.org/10.1093/clinchem/11.4.478
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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