Interference in a radioimmunoassay for human thyrotropin
Abstract
Abnormally high plasma thyrotropin values were found by radioimmunoassay in some patients when an antiserum to porcine thyrotropin was used, normal results being obtained with an antiserum raised to human thyrotropin. These discrepancies were found in some subjects with no biochemical or clinical evidence of hypothyroidism and occasionally in sera from patients with unequivocal hyperthyroidism. We found a substance in serum that cross reacts with the anti-porcine antiserum, is stable on repeated freezing and thawing, and is independent of the 125I-labeled tracer preparation. It is unlikely that this substance is a separation-stage artefact related to immunoglobulins. Its apparent molecular weight (gel filtration) is 114 000, as compared with apparent molecular weights for standard thyrotropin and endogenous thyrotropin (as found in idiopathic hypothyroidism) of 34 700 and 38 000, respectively. We believe the substance is a normal serum constituent that is present in large quantities in a minority of subjects. Apparently unrelated to TSH, its exact chemical nature remains unidentified.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Such interference has been recognised in immunoassays for cholecystokinin, parathyroid hormone, thyrotropic hormone, glucagon, secretin, angiotensin, growth hormone, vasoactive intestinal peptide, gonadotrophins and gonadotrophin-releasing hormone, all of which were resolved by sample extraction.
“Such interference has also been recognised in immunoassays for cholecystokinin 80, parathyroid hormone 81, thyrotropic hormone 82, glucagon 83, secretin 84, 85, angiotensin 86, growth hormone 87, vasoactive intestinal peptide 88, gonadotrophins 89 and gonadotrophin‐releasing hormone 90, all of which were resolved by sample extraction.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [82]
Cited by
Sorted by citations
Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers
Leng, G., & Sabatier, N. (2016) · Journal of Neuroendocrinology · doi:10.1111/jne.12413 · Cited by 237 · see citing works
“Such interference has also been recognised in immunoassays for chole-cystokinin (80), parathyroid hormone (81), thyrotropic hormone (82), glucagon (83), secretin (84,85), angiotensin (86), growth hormone (87), vasoactive intestinal peptide (88), gonadotrophins (89) and gonadotrophin-releasing…”
BackgroundGendrel, D., Feinstein, M.-C., Grenier, J., Roger, M., Ingrand, J., Chaussain, J., Canlorbe, P., & Job, J. C. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-52-1-62 · Cited by 46 · see citing works
HYPERTHYROIDISM DUE TO A PITUITARY TSH SECRETING TUMOUR WITH AMENORRHOEA‐GALACTORRHOEA
Benoit, R., Murphy, B. E. P., Robert, F., Marcovitz, S., Hardy, J., Tsoukas, G. M., & Gardiner, R. (1980) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1980.tb03127.x · Cited by 42 · see citing works
Radioimmunoassay of Human Thyrotropin: Analytical and Clinical Developments
WEHMANN, R. E., & NISULA, B. C. (1984) · CRC Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408368409165776 · Cited by 36 · see citing works
Fifty years of development in the endocrinology laboratory
Lepage, R., & Albert, C. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.03.007 · Cited by 22 · see citing works
Antibody Interference in a Two-Site Immunometnc Assay for Thyrotrophin
John, R., Henley, R., & Barron, N. P. (1989) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456328902600409 · Cited by 15 · see citing works
Thyroid Function Tests in Thyroid and Nonthyroid Disease
Trainer, T. D., Howard, P. L., & Rock, R. C. (1983) · CRC Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408368309165761 · Cited by 13 · see citing works
Nickoloff, E. L., & Demers, L. M. (1984) · CRC Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408368409165784 · Cited by 13 · see citing works
A Monoclonal Antibody-Based Immunoradiometric Assay for h-LH
Hunter, W. M., Bennie, J., Kellett, H. A., Micklem, L. R., Scott, A., & James, K. (1984) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456328402100408 · Cited by 13 · see citing works
Evaluation of thyroid function in patients with thyroid hormone autoantibodies
Sakata, S., Komaki, T., Ogawa, T., Takuno, H., Matsui, I., Sarui, H., KOJIMA, N., Takamatsu, J., & Miura, K. (1993) · Clinica Chimica Acta · doi:10.1016/0009-8981(93)90194-9 · Cited by 7 · see citing works
Rao, C. V., & Hussa, R. O. (1982) · American Journal of Obstetrics and Gynecology · doi:10.1016/s0002-9378(16)32329-8 · Cited by 5 · see citing works
The Role of Immunoassay in the Clinical Laboratory
Nickoloff, E. L. (1986) · Progress in clinical biochemistry and medicine · doi:10.1007/978-3-642-70998-2_4
- Authors
- Spencer, C. A., & Challand, G. S.
- ORCID
- Spencer, C A
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-03-01
- Volume
- 23
- Issue
- 3
- Pages
- 584-588
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1953998315
- Cited by
- 13
- References
- 0
- Citations by year
- 2016: 1
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- thyroid-stimulating hormone, hyperthyroidism, hypothyroidism, molecular weight, immunoglobulins
- MeSH terms
- Animals, Antibody Specificity, Humans, Hypothyroidism, Molecular Weight, Radioimmunoassay, Swine, Thyrotropin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
