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Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
Abstract
A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus. This chromogen system has a high absorptivity, affording useful results with sample/reagent volume ratios as low as 0.025. The procedure is applicable to serum, plasma, or diluted urine. A single working reagent is used; the reaction is complete in less than 15 min at room temperature. The red dye formed is measured at 520 nm; a blank sample measurement is not needed. The standard curve for the method is linear for uric acid concentrations up to 1500 mumol/L. Average analytical recovery of uric acid in human sera and urine exceeded 99%; within-run and between-run precision studies showed CV's of less than or equal to 1.2 and less than or equal to 2.2%, respectively. The new procedure correlated well with the uricase/catalase and uricase/ultraviolet methods. The method is suitable for automation.
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Cited by
Segal, A. W., West, I., Wientjes, F., Nugent, J. H. A., Chavan, A. J., Haley, B., Garcia, R. C., Rosen, H., & Scrace, G. (1992) · Biochemical Journal · doi:10.1042/bj2840781 · Cited by 349
Nelson, S. K., Bose, S. K., Grunwald, G. K., Myhill, P., & McCord, J. M. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.08.043 · Cited by 215
Antioxidant response to oxidative stress induced by exhaustive exercise
Aguiló, A., Tauler, P., Fuentespina, E., Tur, J. A., Córdova, A., & Pons, A. (2005) · Physiology & Behavior · doi:10.1016/j.physbeh.2004.07.034 · Cited by 207
Plasma uric acid and the risk of type 2 diabetes in a Chinese community
Chien, K.-L., Chen, M.-F., Hsu, H.-C., Chang, W.-T., Su, T.-C., Lee, Y.-T., & Hu, F. B. (2008) · Clinical Chemistry · doi:10.1373/clinchem.2007.095190 · Cited by 172
Ibrahim, Y. F., Moussa, R. A., Bayoumi, A. M. A., & Ahmed, A.-S. F. (2020) · Inflammopharmacology · doi:10.1007/s10787-019-00628-y · Cited by 75
Metadata
- Authors
- Fossati, P., Prencipe, L., & Berti, G.
- Venue
- Clinical Chemistry
- Publisher
- American Association for Clinical Chemistry
- Published
- 1980-02-01
- Volume
- 26
- Issue
- 2
- Pages
- 227-231
- ISSN
- 0009-9147 (ISSN-L)
- OpenAlex
- W2162043836
- Cited by
- 1,104
- References
- 0
- FWCI
- 6.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. https://app.cochat.ai/papers/10.1093/clinchem/26.2.0227
- The paper
- Fossati, P., Prencipe, L., & Berti, G. (1980). Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clinical Chemistry. https://doi.org/10.1093/clinchem/26.2.0227
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
