Antioxidant response to oxidative stress induced by exhaustive exercise
Abstract
The aim of this work was to demonstrate the occurrence of oxidative stress during exhaustive exercise and to determine the antioxidant response. Eight voluntary male subjects participated in this study. The exercise was a cycling mountain stage (171 km) and the cyclists took a mean+/-S.E.M. time of 270+/-12 min to complete it. Blood samples were taken before the cycling stage, immediately after the stage, 3 h after finishing the stage and on the morning of the following day. We determined the activities of erythrocyte antioxidant enzymes, blood levels of oxidised glutathione, plasma levels of antioxidant vitamins and carotenoids, and the serum lipid and cholesterol profile. The mountain cycling stage induced significant increases in catalase and glutathione reductase activities. Significant decreases in glutathione peroxidase activity, both determined with hydrogen peroxide and with cumene hydroperoxide as substrates, were observed. Blood oxidised glutathione and serum uric acid rose after the stage. Plasma vitamin E increased after the stage but dropped to below basal values after 3 h of recovery. Triglycerides and VLDL-cholesterol increased significantly after the stage and remained high 3 h after the cycling stage. The mountain cycling stage induced oxidative stress, as was evidenced by the increases in blood GSSG and in serum urate concentrations and by the pattern of change of erythrocyte antioxidant enzyme activities. A specific utilisation of alpha-tocopherol against oxidative stress during recovery was evidenced.
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Extreme exercise disrupts metabolic and physiological processes and leads to impaired cognitive performance in humans.
“Extreme exercise has been shown to disrupt a number of metabolic and physiological processes and lead to impaired cognitive performance in humans (Aguiló et al., 2005).”
From Exercise-Mediated Neurogenesis in the Hippocampus via BDNF (2018) · cites this paper as Aguiló et al., 2005
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- Authors
- Aguiló, A., Tauler, P., Fuentespina, E., Tur, J. A., Córdova, A., & Pons, A.
- Affiliations
- Universitat de les Illes Balears (ES)
- Hospital Universitario Son Dureta (ES)
- Universidad de Valladolid (ES)
- Corresponding author
- Pons, Antoni
- Venue
- Physiology & Behavior
- Publisher
- Elsevier BV
- Published
- 2005-01
- Volume
- 84
- Issue
- 1
- Pages
- 1-7
- ISSN
- 0031-9384 (ISSN-L)
- OpenAlex
- W2086633683
- Cited by
- 207
- References
- 46
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 9 · 2019: 11 · 2018: 8 · 2017: 11 · 2016: 14 · 2015: 14 · 2014: 20 · 2013: 9 · 2012: 7
- FWCI
- 4.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Physiology & Behavior (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- oxidative stress, exhaustive exercise, antioxidant response, erythrocyte antioxidant enzymes, catalase, glutathione reductase, glutathione peroxidase, glutathione disulfide, serum uric acid, vitamin E, carotenoids, lipid profile, triglycerides, VLDL cholesterol, mountain biking, post-exercise recovery
- MeSH terms
- Adult, Analysis of Variance, Antioxidants, Carotenoids, Cholesterol, Chromatography, High Pressure Liquid, Glutathione Peroxidase, Glutathione Reductase, Humans, Lipids, Male, Time Factors, Uric Acid, Vitamins, Exercise, Oxidative Stress, Glutathione Disulfide
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