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Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
Abstract
In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycerol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is monitored in the presence of horseradish peroxidase with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as the chromogenic system. The high absorbance of this chromogen system at 510 nm affords useful results with a sample/reagent volume ratio as low as 1:150, and a blank sample measurement is not needed. A single, stable working reagent is used; the reaction is complete in 15 min at room temperature. The standard curve is linear for triglyceride concentrations as great as 13.6 mmol/L. Average analytical recovery of triglycerides in human sera is 100.1%, and within-run and between-run precision studies showed CVs of less than or equal to 1.6 and less than or equal to 3.0%, respectively. The method is suitable for automation.
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Cited by
Barker, D. J. P., Hales, C. N., Fall, C. H. D., Osmond, C., Phipps, K., & Clark, P. M. S. (1993) · Diabetologia · doi:10.1007/bf00399095 · Cited by 2,468
Trajectories of growth among children who have coronary events as adults
Barker, D. J. P., Osmond, C., Forsén, T. J., Kajantie, E., & Eriksson, J. G. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa044160 · Cited by 1,517
Lemieux, I., Pascot, A., Couillard, C., Lamarche, B., Tchernof, A., Alméras, N., Bergeron, J., Gaudet, D., Tremblay, G., Prud’homme, D., et al. (2000) · Circulation · doi:10.1161/01.cir.102.2.179 · Cited by 937
Safety and Efficacy of Bempedoic Acid to Reduce LDL Cholesterol
Ray, K. K., Bays, H. E., Catapano, A. L., Lalwani, N. D., Bloedon, L. T., Sterling, L. R., Robinson, P. L., & Ballantyne, C. M. (2019) · New England Journal of Medicine · doi:10.1056/nejmoa1803917 · Cited by 807
Antidiabetic effects of quercetin in streptozocin-induced diabetic rats
Vessal, M., Hemmati, M., & Vasei, M. (2003) · Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology · doi:10.1016/s1532-0456(03)00140-6 · Cited by 624
Metadata
- Authors
- Fossati, P., & Prencipe, L.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1982-10-01
- Volume
- 28
- Issue
- 10
- Pages
- 2077-2080
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2154774609
- Cited by
- 2,905
- References
- 0
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fossati, P., & Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry. https://doi.org/10.1093/clinchem/28.10.2077
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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