Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.
Abstract
This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.
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CETP activity was analysed as the transfer/exchange of radiolabelled [14C]cholesteryl oleate between exogenously added human LDL and HDL2, as described in the cited protocol.
“CETP activity was analysed as the transfer/exchange of radiolabelled [14C]cholesteryl oleate between exogenously added human LDL and HDL2, as described in89.”
From Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses (2016) · cites this paper as [89]
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- Authors
- Groener, J. E., Pelton, R. W., & Kostner, G. M.
- ORCID
- Kostner, G M
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1986-02-01
- Volume
- 32
- Issue
- 2
- Pages
- 283-286
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1917789100
- Cited by
- 162
- References
- 0
- Citations by year
- 2025: 1 · 2021: 1 · 2019: 1 · 2017: 3 · 2016: 3 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Keywords
- cholesteryl ester transfer, serum lipoproteins, HDL cholesterol, LDL cholesterol, VLDL, polyethylene glycol precipitation, dextran sulfate precipitation, assay interference, triglycerides
- MeSH terms
- Cholesterol Esters, Humans, Isotope Labeling, Lipoproteins, HDL, Lipoproteins, LDL, Cholesterol, LDL, Lipoproteins, VLDL, Mathematics, Polyethylene Glycols, Chemical Precipitation, Scintillation Counting, Ultracentrifugation
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