Cited by 162Cholesterol and Lipid MetabolismLipid metabolism and disordersLipoproteins and Cardiovascular Health

Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.

Groener, J E, Pelton, R W, Kostner, G M·1986·Clinical Chemistry

Abstract

This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.

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Crossref · 10.1093/clinchem/32.2.283

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Authors
Groener, J. E., Pelton, R. W., & Kostner, G. M.
Venue
Clinical Chemistry
Published
1986-02-01
Volume
32
Issue
2
Pages
283-286
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
OpenAlex
W1917789100
Cited by
162
References
0
Citations by year
2025: 1 · 2021: 1 · 2019: 1 · 2017: 3 · 2016: 3 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
4.00
Type
journal article
Language
en
Open access
closed
Locations
  • Clinical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
Keywords
cholesteryl ester transfer, serum lipoproteins, HDL cholesterol, LDL cholesterol, VLDL, polyethylene glycol precipitation, dextran sulfate precipitation, assay interference, triglycerides
MeSH terms
Cholesterol Esters, Humans, Isotope Labeling, Lipoproteins, HDL, Lipoproteins, LDL, Cholesterol, LDL, Lipoproteins, VLDL, Mathematics, Polyethylene Glycols, Chemical Precipitation, Scintillation Counting, Ultracentrifugation

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