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Heterophilic antibodies: a problem for all immunoassays.
Abstract
We verified that antibody-binding substances in serum that interfere in two-site immunoassays involving murine antibodies are heterophilic antibodies. Incubation of serum containing heterophilic antibodies and a murine monoclonal antibody to human choriogonadotropin (hCG) leads to formation of a series of soluble immune complexes. We investigated the recognition of hCG by reagent antibody in the presence of heterophilic antibodies and found this recognition to be diminished. Consequently, about 30% of serum samples containing heterophilic antibodies falsely appear to contain increased concentrations of hCG. The effect on analyte recognition probably results from steric inhibition of hCG binding to complexed antibody. Heterophilic antibodies detected with a murine antibody also bound immunoglobulin from several other species but did not bind all of those tested.
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Cited by
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Tighe, P. J., Ryder, R. R., Todd, I., & Fairclough, L. C. (2015) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201400130 · Cited by 464
The sick euthyroid syndrome: changes in thyroid hormone serum parameters and hormone metabolism
Docter, R., Krenning, E. P., de Jong, M., & Hennemann, G. (1993) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1993.tb02401.x · Cited by 345
Antibody interference in thyroid assays: a potential for clinical misinformation
Després, N., & Grant, A. M. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.3.440 · Cited by 262
Carvalho, V. M. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.08.027 · Cited by 90
Metadata
- Authors
- Boscato, L. M., & Stuart, M. C.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-01-01
- Volume
- 34
- Issue
- 1
- Pages
- 27-33
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2097122290
- Cited by
- 300
- References
- 0
- FWCI
- 7.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- Boscato, L. M., & Stuart, M. C. (1988). Heterophilic antibodies: a problem for all immunoassays. Clinical Chemistry. https://doi.org/10.1093/clinchem/34.1.27
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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