The coming of age of liquid chromatography coupled to tandem mass spectrometry in the endocrinology laboratory
Abstract
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been rapidly incorporated in the routine of the endocrinology laboratory. Most endocrinologists are aware of the benefits afforded by this technique and tandem mass spectrometers are clearly no longer a mere research method but an important tool widely used for diagnosis. In the last 15 years, LC-MS/MS has replaced techniques such as immunoassay and HPLC for the analysis of hormones because it provides higher specificity and good sensitivity. Also, it permits simultaneous measurement of several analytes and sample preparation and acquisition are fast and simple. Although several strategies based on LC-MS/MS have been described in the last 15 years, there is still room for improvement. The impact of matrix effects and isobaric interferences have been addressed by only a few studies, and standardization with reference materials is available for a limited number of analytes. This review summarizes the application of LC-MS/MS in analyzing three classes of hormones: steroids, derivatives of the aromatic amino acids, and peptides and proteins. The benefits and current limitations of LC-MS/MS will be discussed for these hormone categories.
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Additional factors that may account for variability include specimen handling and preparation, calibration methods, specimen commutability, and interference from matrix material used to store and transport testosterone preparations.
“Additional factors, which may account for variability of results include specimen handling and preparation, calibration methods utilized, specimen commutability, and interference from the matrix material used to store and transport sample T preparations.”
From Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials (2016) · cites this paper as [90]
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- Authors
- Carvalho, V. M.
- Affiliations
- Fleury S.A. (Brazil) (BR)
- Corresponding author
- Carvalho, Valdemir Melechco
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 883-884
- Pages
- 50-58
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W1991871258
- Cited by
- 90
- References
- 156
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 8 · 2021: 1 · 2020: 7 · 2019: 8 · 2018: 7 · 2017: 5 · 2016: 7 · 2015: 8 · 2014: 7 · 2013: 12 · 2012: 6
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- liquid chromatography-tandem mass spectrometry, hormone analysis, steroids, matrix effects, isobaric interference, reference materials
- MeSH terms
- Chemistry, Clinical, Chromatography, Liquid, Endocrinology, Hormones, Humans, Tandem Mass Spectrometry
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