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Development and In Vitro Characterization of a New Immunoassay of Cardiac Troponin T
Abstract
We describe a new one-step enzyme immunoassay of troponin T that uses two specific monoclonal antibodies and streptavidin-coated tubes as the solid phase. The monoclonal antibodies were obtained by conventional hybridoma technology, with human troponin T as antigen. The identity of the cardiac troponin T antigen was confirmed by analysis of the amino acid composition and by partial sequence analysis. The specificity of the monoclonal antibodies for cardiac troponin T was proved by immunoblot analysis and displacement curves. The capture antibody is labeled with biotin. The second antibody is conjugated to horseradish peroxidase (EC 1.11.1.7). The assay [Enzymun-TestR system (Boehringer Mannheim GmbH)] is performed in only 90 min at room temperature; the measuring range for troponin T is 0.1 to 15 micrograms/L. The assay shows excellent between-run precision (CV = 3.3-4.9%).
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Cited by
Biochemical markers of myocardial injury. Is MB creatine kinase the choice for the 1990s?
Adams, J. E., Abendschein, D. R., & Jaffe, A. S. (1993) · Circulation · doi:10.1161/01.cir.88.2.750 · Cited by 554
Cardiac Troponin T in Patients With Clinically Suspected Myocarditis
Lauer, B., Niederau, C., Kühl, U., Schannwell, M., Pauschinger, M., Strauer, B.-E., & Schultheiss, H.-P. (1997) · Journal of the American College of Cardiology · doi:10.1016/s0735-1097(97)00317-3 · Cited by 477
Heeschen, C., Hamm, C. W., Bruemmer, J., & Simoons, M. L. (2000) · Journal of the American College of Cardiology · doi:10.1016/s0735-1097(00)00581-7 · Cited by 414
High-sensitivity assays for troponin in patients with cardiac disease
Westermann, D., Neumann, J. T., Sörensen, N. A., & Blankenberg, S. (2017) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2017.48 · Cited by 222
Metadata
- Authors
- Katus, H. A., Looser, S., Hallermayer, K., Remppis, A., Scheffold, T., Borgya, A., Essig, U., & Geuss, U.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-03-01
- Volume
- 38
- Issue
- 3
- Pages
- 386-393
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1947889433
- Cited by
- 430
- References
- 0
- FWCI
- 23.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- Katus, H. A., Looser, S., Hallermayer, K., Remppis, A., Scheffold, T., Borgya, A., Essig, U., & Geuss, U. (1992). Development and In Vitro Characterization of a New Immunoassay of Cardiac Troponin T. Clinical Chemistry. https://doi.org/10.1093/clinchem/38.3.386
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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