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Plasma amino acids determined by liquid chromatography within 17 minutes
Abstract
We present an HPLC method for the determination of amino acids in plasma. The method is based on automated precolumn derivatization of amino acids with o-phthalaldehyde, separation of the derivatives by reversed-phase chromatography, and quantification by fluorescence detection. Complete separation was achieved within 12 min. Total analysis time, including derivatization, chromatography, and reequilibration of the column, was 17 min. The assay was linear from 5 to 800 mumol/L for all amino acids. Recovery of amino acids added to plasma samples was 96-106%, except for tryptophan (89%). Within-run precision (CV) was 1.8-6.4%, and between-run precision was 2.1-7.2%. The method can be used for determining primary amino acids in plasma and cerebrospinal fluid. The simple sample preparation and short analysis time make the method particularly suitable for routine analysis of large series of samples.
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Cited by
Houdijk, A. P., Rijnsburger, E. R., Jansen, J., Wesdorp, R. I., Weiss, J. K., McCamish, M. A., Teerlink, T., Meuwissen, S. G., Haarman, H. J. T. M., Thijs, L. G., et al. (1998) · The Lancet · doi:10.1016/s0140-6736(98)02007-8 · Cited by 620
Self-citationTeerlink, T., Nijveldt, R. J., de Jong, S., & van Leeuwen, P. A. M. (2002) · Analytical Biochemistry · doi:10.1006/abio.2001.5575 · Cited by 417
Self-citationGarrel, D., Patenaude, J., Nedelec, B., Samson, L., Dorais, J., Champoux, J., D’Elia, M., & Bernier, J. (2003) · Critical Care Medicine · doi:10.1097/01.ccm.0000084848.63691.1e · Cited by 293
Hirche, F., Schröder, A., Knoth, B., Stangl, G. I., & Eder, K. (2006) · British Journal Of Nutrition · doi:10.1079/bjn20061729 · Cited by 68
Protein and amino acid status before and after bariatric surgery: A 12-month follow-up study
Ferreira Nicoletti, C., Morandi Junqueira-Franco, M. V., dos Santos, J. E., Sergio Marchini, J., Junior, W. S., & Nonino, C. B. (2013) · Surgery for Obesity and Related Diseases · doi:10.1016/j.soard.2013.07.004 · Cited by 58
Metadata
- Authors
- Terrlink, T., van Leeuwen, P. A., & Houdijk, A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-02-01
- Volume
- 40
- Issue
- 2
- Pages
- 245-249
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1908510719
- Cited by
- 197
- References
- 0
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Terrlink, T., van Leeuwen, P. A., & Houdijk, A. (1994). Plasma amino acids determined by liquid chromatography within 17 minutes. Clinical Chemistry. https://doi.org/10.1093/clinchem/40.2.245
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
