The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
Open accessAbstract
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
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“dPCR requires the same careful assay design considerations as those required for qPCR (53, 54).”
MethodSelf-citationHigh-throughput droplet digital PCR system for absolute quantitation of DNA copy number
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“Restriction digestion to small linear DNA fragments may equalize template differences (36, 49, 64), and prevent underestimation of linked target molecules (18).”
BackgroundSelf-citationProperties of the Reverse Transcription Reaction in mRNA Quantification
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Cited by
Microglia states and nomenclature: A field at its crossroads
Paolicelli, R. C., Sierra, A., Stevens, B., Tremblay, M.-E., Aguzzi, A., Ajami, B., Amit, I., Audinat, E., Bechmann, I., Bennett, M., et al. (2022) · Neuron · doi:10.1016/j.neuron.2022.10.020 · Cited by 1,973
Graham, K. E., Loeb, S. K., Wolfe, M. K., Catoe, D., Sinnott-Armstrong, N., Kim, S., Yamahara, K. M., Sassoubre, L. M., Mendoza Grijalva, L. M., Roldan-Hernandez, L., et al. (2021) · Environmental Science & Technology · doi:10.1021/acs.est.0c06191 · Cited by 363
Metadata
- Authors
- The dMIQE Group, Whale, A. S., De Spiegelaere, W., Trypsteen, W., Nour, A. A., Bae, Y.-K., Benes, V., Burke, D., Cleveland, M., Corbisier, P., Devonshire, A. S., Dong, L., Drandi, D., Foy, C. A., Garson, J. A., He, H.-J., Hellemans, J., Kubista, M., Lievens, A., Makrigiorgos, M. G., Milavec, M., Mueller, R. D., Nolan, T., O’Sullivan, D. M., Pfaffl, M. W., Rödiger, S., Romsos, E. L., Shipley, G. L., Taly, V., Untergasser, A., Wittwer, C. T., Bustin, S. A., Vandesompele, J., & Huggett, J. F.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-08-01
- Volume
- 66
- Issue
- 8
- Pages
- 1012-1029
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W3047062195
- Cited by
- 516
- References
- 11
- FWCI
- 17.63
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- The dMIQE Group, Whale, A. S., De Spiegelaere, W., Trypsteen, W., Nour, A. A., Bae, Y.-K., Benes, V., Burke, D., Cleveland, M., Corbisier, P., Devonshire, A. S., Dong, L., Drandi, D., Foy, C. A., Garson, J. A., He, H.-J., Hellemans, J., Kubista, M., Lievens, A., . . . Huggett, J. F. (2020). The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. Clinical Chemistry. https://doi.org/10.1093/clinchem/hvaa125
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
