MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes
Open accessAbstract
Defects of the myosin VIIa motor protein cause deafness and retinal anomalies in humans and mice. We report on the identification of a novel myosin-VIIa-interacting protein that we have named MyRIP (myosin-VIIa- and Rab-interacting protein), since it also binds to Rab27A in a GTP-dependent manner. In the retinal pigment epithelium cells, MyRIP, myosin VIIa and Rab27A are associated with melanosomes. In transfected PC12 cells, overexpression of MyRIP was shown to interfere with the myosin VIIa tail localization. We propose that a molecular complex composed of Rab27A, MyRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosinVIIa-defective mice.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Myosin-7a associates with RPE melanosomes by binding to MYRIP, which in turn binds to RAB27A on the melanosome surface.
“myosin-7a associates with RPE melanosomes by binding to myosin-7a- and Rab-interacting protein (MYRIP) (El-Amraoui et al., 2002), which in turn binds to RAB27A on the melanosome surface (Futter et al., 2004; Gibbs et al., 2004; Kuroda and Fukuda, 2005; Klomp et al., 2007)”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as El-Amraoui et al., 2002
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Mutations in RAB27A cause Griscelli syndrome associated with haemophagocytic syndrome
Ménasché, G., Pastural, É., Feldmann, J., Dogniaux, S., Ersoy, F., Dupuis, S., Wulffraat, N. M., Bianchi, D. W., Fischer, A., Deist, F. L., et al. (2000) · Nature Genetics · doi:10.1038/76024 · Cited by 957 · see citing works
Novel myosin heavy chain encoded by murine dilute coat colour locus
Mercer, J. A., Seperack, P. K., Strobel, M. C., Copeland, N. G., & Jenkins, N. A. (1991) · Nature · doi:10.1038/349709a0 · Cited by 536 · see citing works
The melanosome: membrane dynamics in black and white
Marks, M. S., & Seabra, M. C. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35096009 · Cited by 452 · see citing works
Hasson, T., Heintzelman, M. B., Santos-Sacchi, J., Corey, D. P., & Mooseker, M. S. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.21.9815 · Cited by 427 · see citing works
A mutation in Rab27a causes the vesicle transport defects observed in ashen mice
Wilson, S. M., Yip, R., Swing, D. A., O’Sullivan, T. N., Zhang, Y., Novak, E. K., Swank, R. T., Russell, L. B., Copeland, N. G., & Jenkins, N. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.140212797 · Cited by 406 · see citing works
Ostermeier, C., & Brunger, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80549-8 · Cited by 363 · see citing works
Rab27a Regulates the Peripheral Distribution of Melanosomes in Melanocytes
Hume, A. N., Collinson, L., Rapak, A., Gomes, A. Q., Hopkins, C. R., & Seabra, M. C. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.4.795 · Cited by 333 · see citing works
Self, T. J., Mahony, M., Fleming, J., Walsh, J. C., Brown, S. D. M., & Steel, K. P. (1998) · Development · doi:10.1242/dev.125.4.557 · Cited by 319 · see citing works
Myosin VIIa Participates in Opsin Transport through The Photoreceptor Cilium
Liu, X., Udovichenko, I. P., Brown, S. D. M., Steel, K. P., & Williams, D. S. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-15-06267.1999 · Cited by 294 · see citing works
U SHER S YNDROME : From Genetics to Pathogenesis
Petit, C. (2001) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.2.1.271 · Cited by 280 · see citing works
Self-citationMatesic, L. E., Yip, R., Reuss, A. E., Swing, D. A., O’Sullivan, T. N., Fletcher, C., Copeland, N. G., & Jenkins, N. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.181336698 · Cited by 258 · see citing works
Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle
Wu, X., Rao, K., Bowers, M., Copeland, N. G., Jenkins, N. A., & Hammer, J. A. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.6.1091 · Cited by 233 · see citing works
Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin–catenins complex
Küssel‐Andermann, P., El‐Amraoui, A., Safieddine, S., Nouaille, S., Perfettini, I., Lecuit, M., Cossart, P., Uwe Wolfrum, B., & Petit, C. (2000) · The EMBO Journal · doi:10.1093/emboj/19.22.6020 · Cited by 229 · see citing works
Self-citationBahadoran, P., Aberdam, E., Mantoux, F., Buscà, R., Bille, K., Yalman, N., de Saint-Basile, G., Casaroli-Marano, R., Ortonne, J.-P., & Ballotti, R. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.4.843 · Cited by 221 · see citing works
Kuroda, T. S., Fukuda, M., Ariga, H., & Mikoshiba, K. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112414200 · Cited by 211 · see citing works
Mutant myosin VIIa causes defective melanosome distribution in the RPE of shaker-1 mice
Liu, X., Ondek, B., & Williams, D. S. (1998) · Nature Genetics · doi:10.1038/470 · Cited by 168 · see citing works
el-Amraoui, A. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.8.1171 · Cited by 158 · see citing works
Self-citationTwo Genes Are Responsible for Griscelli Syndrome at the Same 15q21 Locus
Pastural, É., Ersoy, F., Yalman, N., Wulffraat, N. M., Grillo, E., Özkınay, F., Tezcan, İ., Gedikoğlu, G., Philippe, N., Fischer, A., et al. (2000) · Genomics · doi:10.1006/geno.1999.6081 · Cited by 120 · see citing works
Recurring views on the structure and function of the cytoskeleton: A 300-Year Epic
Frixione, E. (2000) · Cell Motility and the Cytoskeleton · doi:10.1002/1097-0169(200006)46:2 · Cited by 85 · see citing works
Unconventional Myosin VIIA Is a Novel A-kinase-anchoring Protein
Küssel‐Andermann, P., El-Amraoui, A., Safieddine, S., Hardelin, J., Nouaille, S., Camonis, J., & Petit, C. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m004393200 · Cited by 63 · see citing works
Self-citationWang, X., Hu, B., Zimmermann, B., & Kilimann, M. W. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103337200 · Cited by 59 · see citing works
Haynes, L. P., Evans, G. J., Morgan, A., & Burgoyne, R. D. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006959200 · Cited by 47 · see citing works
Rab3a Binding and Secretion-enhancing Domains in Rim1 Are Separate and Unique
Sun, L., Bittner, M. A., & Holz, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011110200 · Cited by 33 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The cell biology of the retinal pigment epithelium
Lakkaraju, A., Umapathy, A., Tan, L. X., Daniele, L., Philp, N. J., Boesze-Battaglia, K., & Williams, D. S. (2020) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100846 · Cited by 422 · see citing works
Gibbs, D., Kitamoto, J., & Williams, D. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1130432100 · Cited by 250 · see citing works
Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes
Gibbs, D., Azarian, S. M., Lillo, C., Kitamoto, J., Klomp, A. E., Steel, K. P., Libby, R. T., & Williams, D. S. (2004) · Journal of Cell Science · doi:10.1242/jcs.01580 · Cited by 152 · see citing works
Futter, C. E., Ramalho, J. S., Jaissle, G. B., Seeliger, M. W., & Seabra, M. C. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-10-0772 · Cited by 110 · see citing works
Lopes, V. S., Ramalho, J. S., Owen, D. M., Karl, M. O., Strauss, O., Futter, C. E., & Seabra, M. C. (2007) · Traffic · doi:10.1111/j.1600-0854.2007.00548.x · Cited by 95 · see citing works
Proteomic analysis of mature melanosomes from the retinal pigmented epithelium
Azarian, S. M., McLeod, I., Lillo, C., Gibbs, D., Yates, J. R., & Williams, D. S. (2006) · Journal of Proteome Research · doi:10.1021/pr0502323 · Cited by 35 · see citing works
McNeil, E. L., Tacelosky, D., Basciano, P., Biallas, B., Williams, R., Damiani, P., Deacon, S., Fox, C., Stewart, B., Petruzzi, N., et al. (2004) · Cell Motility · doi:10.1002/cm.10179 · Cited by 20 · see citing works
- Authors
- El‐Amraoui, A., Schonn, J., Küssel‐Andermann, P., Blanchard, S., Desnos, C., Henry, J., Wolfrum, U., Darchen, F., & Petit, C.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Petit, Christine
- Venue
- The EMBO Reports
- Published
- 2002-05-01
- Volume
- 3
- Issue
- 5
- Pages
- 463-470
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2096369586
- Cited by
- 187
- References
- 23
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 11 · 2019: 1 · 2018: 2 · 2017: 6 · 2016: 4 · 2015: 2 · 2014: 7 · 2013: 5 · 2012: 5
- FWCI
- 7.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- EMBO Reports (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- MYO7A, Rab27a, retinal pigment epithelium, melanosomes, Rab GTPase effectors, actin cytoskeleton, Usher syndrome, Usher syndrome type 1B, deafness, organelle transport
- MeSH terms
- rab27 GTP-Binding Proteins, Myosin VIIa, Amino Acid Sequence, Animals, Dyneins, Humans, Molecular Sequence Data, Myosins, Organ Specificity, Retina, Gene Library, Sequence Alignment, Melanosomes, rab GTP-Binding Proteins, Two-Hybrid System Techniques, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
