Proteomic analysis of mature melanosomes from the retinal pigmented epithelium
Abstract
The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosomal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.
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RPE melanosomes contain proteases including cathepsin D and have been observed to fuse with phagosomes, suggesting they may contribute to degradation of the phagocytic load.
“RPE melanosomes contain proteases (Azarian et al., 2006), including cathepsin D, a major enzyme in the degradation of OS proteins (Hayasaka et al., 1975), and have been observed to fuse with phagosomes (e.g. Schraermeyer et al., 1999).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Azarian et al., 2006
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The cell biology of the retinal pigment epithelium
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- Authors
- Azarian, S. M., McLeod, I., Lillo, C., Gibbs, D., Yates, J. R., & Williams, D. S.
- Affiliations
- Scripps Research Institute (US)
- Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, California 92093-0912, and Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037
- Corresponding author
- Williams, David S.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-01-31
- Volume
- 5
- Issue
- 3
- Pages
- 521-529
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2052526264
- Cited by
- 35
- References
- 31
- Citations by year
- 2026: 1 · 2022: 1 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 1
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p30ey012598-05)
- National Eye Institute (R01 EY07042, EY 12598, R01EY007042)
- National Center for Research Resources (P41 RR 11823, P41RR011823)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Keywords
- melanosomes, retinal pigment epithelium, lysosomal enzymes, cathepsin D
- MeSH terms
- Animals, Cells, Cultured, Epithelial Cells, Pigment Epithelium of Eye, Retina, Mass Spectrometry, Swine, Microscopy, Immunoelectron, Melanosomes, Proteome
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