The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Wnt pathway activation, cell migration, and lipid uptake is regulated by low-density lipoprotein receptor-related protein 5 in human macrophages
Abstract
Aims Atherosclerosis plaque development includes infiltration of inflammatory cells, accumulation of lipids and fibrous cap formation. Low-density lipoprotein receptor-related protein 1 (LRP1) is expressed on atherosclerotic lesions associated with macrophages and vascular smooth muscle cells. The aim of this work is to analyse the role in atherosclerosis lesion progression of another member of the LDL receptor protein family, low-density lipoprotein receptor-related protein 5 (LRP5), a co-receptor with Frizzled known to activate the Wnt signalling pathway in several cell types. Methods and results LRP5 is expressed in human vascular and innate inflammatory cells. LRP5 is transcriptionally regulated by aggregated LDL (agLDL), participating in the lipid uptake and transformation of macrophages into foam cells, a critical step in atherosclerosis progression. AgLDL-treated macrophages show up-regulated expression of β-catenin, LEF1, c-jun, cyclinD1, bone morphogenetic protein 2 (BMP2), and osteopontin (OPN), proteins and targets of the Wnt signalling pathway, whereas LRP5-silenced macrophages show a significant down-regulation of OPN and BMP2 expression. Furthermore, LRP5-deficient macrophages exhibit an impaired migration both in wound-repair and modified Boyden chambers models. Conclusion These results demonstrate the involvement of LRP5 in the innate inflammatory reaction to lipid infiltration in atherosclerosis.
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Increased LRP5 expression is induced by extracellular lipids in primary cultures of human monocytes and macrophages and human vascular smooth muscle cells and in vivo in ApoE-/- mice.
“Increased LRP5 expression is induced by extracellular lipids in the primary cultures of human monocytes and macrophages and human vascular smooth muscle cells 3 and in vivo in ApoE -/-mice.26”
From Canonical Wnt pathway and the LDL receptor superfamily in neuronal cholesterol homeostasis and function (2024) · cites this paper as [3]
In the adult, LRPs are involved in the clearance of cholesterol and cholesteryl ester-containing LDL particles from the blood.
“In the adult, LRPs are involved for their role in the clearance of cholesterol and cholesteryl ester-containing LDL particles from the blood.3”
From Canonical Wnt pathway and the LDL receptor superfamily in neuronal cholesterol homeostasis and function (2024) · cites this paper as [3]
Macrophages that do not express LRP5 show less intracellular cholesterol ester accumulation than Wt macrophages.
“Also, macrophages that do not express LRP5 show less intracellular cholesterol ester accumulation than Wt macrophages.3”
From Canonical Wnt pathway and the LDL receptor superfamily in neuronal cholesterol homeostasis and function (2024) · cites this paper as [3]
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Canonical Wnt signaling is antagonized by noncanonical Wnt5a in hepatocellular carcinoma cells
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Borrell-Pages, M., Luquero, A., Vilahur, G., Padró, T., & Badimon, L. (2024) · Cardiovascular Research · doi:10.1093/cvr/cvad159 · Cited by 10
Metadata
- Authors
- Borrell-Pagès, M., Romero, J. C., Juan-Babot, O., & Badimon, L.
- Venue
- European Heart Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-03-12
- Volume
- 32
- Issue
- 22
- Pages
- 2841-2850
- ISSN
- 1522-9645
- 0195-668X
- Cited by
- 84
- References
- 2
- Language
- en
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