The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Genetic applications of an inverse polymerase chain reaction.
Open accessAbstract
A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
Jiang, Y., Chen, B., Duan, C., Sun, B., Yang, J., & Yang, S. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.04023-14 · Cited by 1,362
Liu, Y.-G., & Whittier, R. F. (1995) · Genomics · doi:10.1016/0888-7543(95)80010-j · Cited by 1,123
Multipotent neural cell lines can engraft and participate in development of mouse cerebellum
Snyder, E. Y., Deitcher, D. L., Walsh, C., Arnold-Aldea, S., Hartwieg, E. A., & Cepko, C. L. (1992) · Cell · doi:10.1016/0092-8674(92)90204-p · Cited by 915
Kohn, D. B., Weinberg, K. I., Nolta, J. A., Heiss, L. N., Lenarsky, C., Crooks, G. M., Hanley, M. E., Annett, G., Brooks, J. S., El-Khoureiy, A., et al. (1995) · Nature Medicine · doi:10.1038/nm1095-1017 · Cited by 594
Molecular Evidence for the Early Evolution of Photosynthesis
Xiong, J., Fischer, W. M., Inoue, K., Nakahara, M., & Bauer, C. E. (2000) · Science · doi:10.1126/science.289.5485.1724 · Cited by 513
Metadata
- Authors
- Ochman, H., Gerber, A. S., & Hartl, D. L.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-11-01
- Volume
- 120
- Issue
- 3
- Pages
- 621-623
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2159686532
- Cited by
- 1,859
- References
- 2
- FWCI
- 10.93
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ochman, H., Gerber, A. S., & Hartl, D. L. (1988). Genetic applications of an inverse polymerase chain reaction. Genetics. https://doi.org/10.1093/genetics/120.3.621
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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