Spontaneous mutation in Escherichia coli containing the dnaE911 DNA polymerase antimutator allele
Open accessAbstract
We have previously isolated mutants of Escherichia coli that replicate their DNA with increased fidelity. These mutants have a mutation in the dnaE gene, encoding the alpha subunit of DNA polymerase III. They were isolated in a mismatch-repair-defective mutL background, in which mutations can be considered to represent uncorrected DNA replication errors. In the present study we analyze the effect of one of these alleles, dnaE911, on spontaneous mutagenesis in a mismatch-repair-proficient background. In this background, spontaneous mutations may be the sum of uncorrected replication errors and mutations resulting from other pathways. Hence, the effect of the dnaE allele may provide insights into the contribution of uncorrected DNA replication errors to spontaneous mutation. The data show that dnaE911 decreases the level of Rifr, lacI and galK mutations in this background by 1.5-2-fold. DNA sequencing of 748 forward mutants in the lacI gene reveals that this effect has a clear specificity. Transversions are decreased by approximately 3-fold, whereas transitions, frameshifts, deletions and duplications remain essentially unchanged. Among the transversions, A.T-->T.A are affected most strongly (approximately 6-fold). In addition to this effect on transversions within the lacI gene, one previously recognized A.T-->G.C base-pair substitution hotspot in the lac operator is also reduced (approximately 5-fold). The data are discussed in the light of the role of DNA replication errors in spontaneous mutation, as well as other possible explanations for the observed antimutator effects.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In growing E. coli populations, mutants of the α subunit of replicative DNA polymerase III have been well characterized as antimutator alleles, suggesting that DNA replication errors are a major source of spontaneous mutagenesis under optimal growth conditions.
“In the growing E. coli population, mutants of the α subunit of replicative DNA polymerase III have been well characterized as antimutator alleles, suggesting that DNA replication errors are a major source of spontaneous mutagenesis under optimal growth conditions [76].”
From DNA Data Storage. (2023) · cites this paper as [76]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli
Walker, G. C. (1984) · Microbiological Reviews · doi:10.1128/mr.48.1.60-93.1984 · Cited by 1,770 · see citing works
Stillman, B. (1995) · Molecular Medicine · doi:10.1007/bf03401885 · Cited by 1,031 · see citing works
MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis
Maki, H., & Sekiguchi, M. (1992) · Nature · doi:10.1038/355273a0 · Cited by 891 · see citing works
MECHANISMS AND BIOLOGICAL EFFECTS OF MISMATCH REPAIR
Modrich, P. L. (1991) · Annual Review of Genetics · doi:10.1146/annurev.ge.25.120191.001305 · Cited by 884 · see citing works
Michaels, M. L., Cruz, C., Grollman, A. P., & Miller, J. H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.15.7022 · Cited by 595 · see citing works
Genetic studies of the lac repressor
Farabaugh, P. J., Schmeissner, U., Hofer, M., & Miller, J. H. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90023-2 · Cited by 517 · see citing works
Cupples, C. G., & Miller, J. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.14.5345 · Cited by 453 · see citing works
The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversions.
Nghiem, Y., Cabrera, M., Cupples, C. G., & Miller, J. H. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.8.2709 · Cited by 343 · see citing works
Studies on the Biochemical Basis of Spontaneous Mutation
Muzyczka, N., Poland, R. L., & Bessman, M. J. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44602-4 · Cited by 342 · see citing works
Schaaper, R. M., Danforth, B. N., & Glickman, B. W. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90509-7 · Cited by 306 · see citing works
Self-citationBase selection, proofreading, and mismatch repair during DNA replication in Escherichia coli.
Schaaper, R. M. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80446-3 · Cited by 305 · see citing works
Self-citationSchaaper, R. M., & Dunn, R. L. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.17.6220 · Cited by 294 · see citing works
Self-citationSpontaneous Mutation: Genetic Control of Mutation Rates in Bacteriophage T4
Drake, J. W., Allen, E. F., Forsberg, S. A., Preparata, R.-M., & Greening, E. O. (1969) · Nature · doi:10.1038/2211128a0 · Cited by 206 · see citing works
Miller, J. H., & Low, K. B. (1984) · Cell · doi:10.1016/0092-8674(84)90400-8 · Cited by 142 · see citing works
Witkin, E. M., McCall, J., Volkert, M. R., & Wermundsen, I. E. (1982) · Molecular and General Genetics MGG · doi:10.1007/bf00333788 · Cited by 124 · see citing works
Rapid repeated cloning of mutant lac repressor genes
Schaaper, R. M., Danforth, B. N., & Glickman, B. W. (1985) · Gene · doi:10.1016/0378-1119(85)90312-9 · Cited by 102 · see citing works
Self-citationMutants of Escherichia coli with increased fidelity of DNA replication.
Fijałkowska, I. J., Dunn, R. L., & Schaaper, R. M. (1993) · Genetics · doi:10.1093/genetics/134.4.1023 · Cited by 89 · see citing works
Self-citationThe lacI Gene: Its Role in lac Operon Control and Its Use as a Genetic SystemPreprint
Miller, J. H. (1980) · Cold Spring Harbor Monograph Archive · doi:10.1101/087969133.7.31 · Cited by 74 · see citing works
Reha-Krantz, L. J., & Nonay, R. L. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37508-7 · Cited by 73 · see citing works
General Antimutators are Improbable
Drake, J. W. (1993) · Journal of Molecular Biology · doi:10.1006/jmbi.1993.1002 · Cited by 66 · see citing works
Transversion mutagenesis in bacteriophage T4
Ripley, L. S. (1975) · Molecular and General Genetics MGG · doi:10.1007/bf00332376 · Cited by 59 · see citing works
Schaaper, R. M., & Dunn, R. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)49248-4 · Cited by 54 · see citing works
Self-citationSpacciapoli, P., & Nossal, N. G. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42370-2 · Cited by 46 · see citing works
Kupchella, E., & Cebula, T. A. (1991) · Environmental and Molecular Mutagenesis · doi:10.1002/em.2850180404 · Cited by 43 · see citing works
Ripley, L. S., Glickman, B. W., & Shoemaker, N. B. (1983) · Molecular and General Genetics MGG · doi:10.1007/bf00326062 · Cited by 24 · see citing works
Oller, A. R., Fijałkowska, I. J., & Schaaper, R. M. (1993) · Mutation Research/Environmental Mutagenesis and Related Subjects · doi:10.1016/0165-1161(93)90145-p · Cited by 19 · see citing works
Self-citationHalliday, J. A., Zieleńska, M., Awadallah, S. S., et al. (1990) · Environmental and Molecular Mutagenesis · doi:10.1002/em.2850160303 · Cited by 17 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- YATAGN, F., J. A. HNLIDAY and B. W. GI ICKMAN, 1991 Specificity of recA441-mediated ( t i f -I ) mutational events. Mol. Cen. Genet. 175-1 85. 268 23762-23765. 43-50. 230 75-80. Communicating editor: P. L. FOSTER Downloaded from https://academic.oup.com/genetics/article/138/2/263/6012698 by guest on 18 July 2024
Cited by
Sorted by citations
Heterogeneity of spontaneous DNA replication errors in single isogenic <i>Escherichia coli</i> cells
Woo, A. C., Faure, L., Dapa, T., & Matic, I. (2018) · Science Advances · doi:10.1126/sciadv.aat1608 · Cited by 29 · see citing works
Suppressors of Escherichia coli mutT: antimutators for DNA replication errors
Schaaper, R. M. (1996) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(95)00086-0 · Cited by 27 · see citing works
Buko, T., Tuczko, N., & Ishikawa, T. (2023) · BioTech · doi:10.3390/biotech12020044 · Cited by 21 · see citing works
- Authors
- Oller, A. R., & Schaaper, R. M.
- Affiliations
- National Institutes of Health (US)
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-10-01
- Volume
- 138
- Issue
- 2
- Pages
- 263-270
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1913707695
- Cited by
- 33
- References
- 27
- Citations by year
- 2025: 2 · 2023: 2 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2014: 4 · 2012: 2
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- DNA polymerase III alpha subunit, spontaneous mutagenesis, Escherichia coli, DNA replication errors, DNA mismatch repair, transversions, LacI, lac operator, rifampicin resistance, galK
- MeSH terms
- Base Sequence, DNA Polymerase III, Escherichia coli, Genes, Bacterial, Genes, Dominant, Genotype, Molecular Sequence Data, Mutation, Oligodeoxyribonucleotides, Frameshift Mutation, Point Mutation, Sequence Deletion
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
