Heterogeneity of spontaneous DNA replication errors in single isogenic Escherichia coli cells
Open accessAbstract
cells growing under optimal conditions without exogenous stress. We found that spontaneous DNA replication errors in proliferating cells arose more frequently in subpopulations experiencing endogenous stresses, such as problems with proteostasis, genome maintenance, and reactive oxidative species production. The presence of these subpopulations of phenotypic mutators is not expected to affect the average mutation frequency or to reduce the mean population fitness in a stable environment. However, these subpopulations can contribute to overall population adaptability in fluctuating environments by serving as a reservoir of increased genetic variability.
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These mutator subpopulations do not significantly impact the average mutation frequency or overall fitness of the population in a stable environment, but play a crucial role in enhancing population adaptability in fluctuating environments by providing a reservoir of increased genetic variability.
“These mutator subpopulations do not significantly impact the average mutation frequency or the overall fitness of the population in a stable environment. However, they play a crucial role in enhancing population adaptability in fluctuating environments by providing a reservoir of increased genetic variability [78].”
From DNA Data Storage. (2023) · cites this paper as [78]
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Experimental — accuracy not yet measuredSpontaneous DNA replication errors occurred more frequently in subpopulations experiencing internal stresses, such as issues with proteostasis, genome maintenance, and reactive oxidative species production.
“It was revealed that spontaneous DNA replication errors occurred more frequently in subpopulations experiencing internal stresses, such as issues with proteostasis, genome maintenance, and reactive oxidative species production.”
From DNA Data Storage. (2023) · cites this paper as [78]
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- Authors
- Woo, A. C., Faure, L., Dapa, T., & Matic, I.
- Affiliations
- Inserm (FR)
- INSERM U1001, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine Paris Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France.
- Venue
- Science Advances
- Published
- 2018-06
- Volume
- 4
- Issue
- 6
- Pages
- eaat1608-eaat1608
- ISSN
- 2375-2548 (ISSN-L)
- PMC
- PMC6010332
- OpenAlex
- W2809302957
- Cited by
- 29
- References
- 40
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 7 · 2023: 4 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 4
- FWCI
- 1.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Science Advances (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (Idex ANR-11-IDEX-0005-01 / ANR-11-LABX-0071)
- Fondation pour la recherche Médicale en France (DBF20160635736)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- mutagenesis, single-cell heterogeneity, endogenous stress, proteostasis, genome maintenance, reactive oxygen species, mutation frequency, population adaptability, genetic variation
- MeSH terms
- Anti-Bacterial Agents, DNA Replication, Escherichia coli, Mutation, Stress, Physiological, Protein Biosynthesis, Gene Expression, Genes, Reporter, Single-Cell Analysis
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