The DOI matches its Crossref record.
High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation
Abstract
We have established a high-efficiency method for transforming the unicellular, green alga Chlamydomonas reinhardtii by electroporation. Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid of argininosuccinate lyase (encoded by ARG7), in the presence of the plasmid pJD67 (which contains ARG7) was used to optimize conditions for the introduction of exogenous DNA. The conditions that were varied included osmolarity, temperature, concentration of exogenous DNA, voltage and capacitance. Following optimization, the maximum transformation frequency obtained was 2 × 105 transformants per μg of DNA; this frequency is two orders of magnitude higher than obtained with the current standard method using glass beads to introduce exogenous DNA. The electroporation procedure described in this article is of general utility, and makes it feasible to isolate genes by direct complementation of Chlamydomonas reinhardtii mutants.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution
Rosenberg, J. N., Oyler, G. A., Wilkinson, L., & Betenbaugh, M. J. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.07.008 · Cited by 631
Microalgae as a future food source
Torres-Tiji, Y., Fields, F. J., & Mayfield, S. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107536 · Cited by 559
National Algal Biofuels Technology Roadmap
USDOE Office of Energy Efficiency and Renewable Energy (EERE), O. O. S. T. B. T. O. (., Ferrell, J., US Department of Energy (USDOE), W., & Sarisky-Reed, V. (2010) · doi:10.2172/1218560 · Cited by 470
Fuhrmann, M., Oertel, W., & Hegemann, P. (1999) · The Plant Journal · doi:10.1046/j.1365-313x.1999.00526.x · Cited by 304
Metadata
- Authors
- Shimogawara, K., Fujiwara, S., Grossman, A., & Usuda, H.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-04-01
- Volume
- 148
- Issue
- 4
- Pages
- 1821-1828
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1907570628
- Cited by
- 487
- References
- 0
- FWCI
- 8.48
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation. https://app.cochat.ai/papers/10.1093/genetics/148.4.1821
- The paper
- Shimogawara, K., Fujiwara, S., Grossman, A., & Usuda, H. (1998). High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation. Genetics. https://doi.org/10.1093/genetics/148.4.1821
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
