The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana
Open accessAbstract
The purpose of this project was to identify large numbers of Arabidopsis genes with essential functions during seed development. More than 120,000 T-DNA insertion lines were generated following Agrobacterium-mediated transformation. Transgenic plants were screened for defective seeds and putative mutants were subjected to detailed analysis in subsequent generations. Plasmid rescue and TAIL-PCR were used to recover plant sequences flanking insertion sites in tagged mutants. More than 4200 mutants with a wide range of seed phenotypes were identified. Over 1700 of these mutants were analyzed in detail. The 350 tagged embryo-defective (emb) mutants identified to date represent a significant advance toward saturation mutagenesis of EMB genes in Arabidopsis. Plant sequences adjacent to T-DNA borders in mutants with confirmed insertion sites were used to map genome locations and establish tentative identities for 167 EMB genes with diverse biological functions. The frequency of duplicate mutant alleles recovered is consistent with a relatively small number of essential (EMB) genes with nonredundant functions during seed development. Other functions critical to seed development in Arabidopsis may be protected from deleterious mutations by extensive genome duplications.
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Cites
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953
Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
Aida, M., Ishida, T., Fukaki, H., Fujisawa, H., & Tasaka, M. (1997) · The Plant Cell · doi:10.1105/tpc.9.6.841 · Cited by 1,486
Cited by
Park, W., Li, J., Song, R., Messing, J., & Chen, X. (2002) · Current Biology · doi:10.1016/s0960-9822(02)01017-5 · Cited by 1,244
A High-Throughput Arabidopsis Reverse Genetics System
Sessions, A., Burke, E., Presting, G., Aux, G., McElver, J., Patton, D., Dietrich, B., Ho, P., Bacwaden, J., Ko, C., et al. (2002) · The Plant Cell · doi:10.1105/tpc.004630 · Cited by 961
Self-citationMengiste, T., Chen, X., Salmeron, J., & Dietrich, R. (2003) · The Plant Cell · doi:10.1105/tpc.014167 · Cited by 553
The development of Arabidopsis as a model plant
Koornneef, M., & Meinke, D. (2010) · The Plant Journal · doi:10.1111/j.1365-313x.2009.04086.x · Cited by 489
Self-citationAbiotic stress tolerance mediated by protein ubiquitination
Lyzenga, W. J., & Stone, S. L. (2012) · Journal of Experimental Botany · doi:10.1093/jxb/err310 · Cited by 428
Metadata
- Authors
- McElver, J., Tzafrir, I., Aux, G., Rogers, R., Ashby, C., Smith, K., Thomas, C., Schetter, A., Zhou, Q., Cushman, M. A., Tossberg, J., Nickle, T., Levin, J. Z., Law, M., Meinke, D., & Patton, D.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-12-01
- Volume
- 159
- Issue
- 4
- Pages
- 1751-1763
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2159342305
- Cited by
- 298
- References
- 3
- FWCI
- 19.87
- Type
- journal article
- Language
- en
- Open access
- bronze
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- McElver, J., Tzafrir, I., Aux, G., Rogers, R., Ashby, C., Smith, K., Thomas, C., Schetter, A., Zhou, Q., Cushman, M. A., Tossberg, J., Nickle, T., Levin, J. Z., Law, M., Meinke, D., & Patton, D. (2001). Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana. Genetics. https://doi.org/10.1093/genetics/159.4.1751
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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