The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
A High-Throughput Arabidopsis Reverse Genetics System
Open accessAbstract
A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymmetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from approximately 100000 transformed lines. A total of 85108 TAIL-PCR products from 52964 T-DNA lines were sequenced and compared with the Arabidopsis genome to determine the positions of T-DNAs in each line. Predicted T-DNA insertion sites, when mapped, showed a bias against predicted coding sequences. Predicted insertion mutations in genes of interest can be identified using Arabidopsis Gene Index name searches or by BLAST (Basic Local Alignment Search Tool) search. Insertions can be confirmed by simple PCR assays on individual lines. Predicted insertions were confirmed in 257 of 340 lines tested (76%). This resource has been named SAIL (Syngenta Arabidopsis Insertion Library) and is available to the scientific community at www.tmri.org.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Base-Calling of Automated Sequencer Traces Using Phred. I. Accuracy Assessment
Ewing, B., Hillier, L., Wendl, M. C., & Green, P. (1998) · Genome Research · doi:10.1101/gr.8.3.175 · Cited by 6,967
Liu, Y., Mitsukawa, N., Oosumi, T., & Whittier, R. F. (1995) · The Plant Journal · doi:10.1046/j.1365-313x.1995.08030457.x · Cited by 1,386
Liu, Y.-G., & Whittier, R. F. (1995) · Genomics · doi:10.1016/0888-7543(95)80010-j · Cited by 1,123
T-DNA as an Insertional Mutagen in Arabidopsis
Krysan, P. J., Young, J. C., & Sussman, M. R. (1999) · The Plant Cell · doi:10.1105/tpc.11.12.2283 · Cited by 731
Nawrath, C., Heck, S., Parinthawong, N., & Métraux, J.-P. (2002) · The Plant Cell · doi:10.1105/tpc.010376 · Cited by 604
DNA damage promotes jumping between templates during enzymatic amplification.
Pääbo, S., Irwin, D. M., & Wilson, A. C. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39621-8 · Cited by 496
Tissier, A. F., Marillonnet, S., Klimyuk, V., Patel, K., Torres, M. A., Murphy, G., & Jones, J. D. G. (1999) · The Plant Cell · doi:10.1105/tpc.11.10.1841 · Cited by 364
T-DNA insertion mutagenesis in Arabidopsis: going back and forth
Azpiroz-Leehan, R. (1997) · Trends in Genetics · doi:10.1016/s0168-9525(97)01094-9 · Cited by 327
T-DNA integration: a mode of illegitimate recombination in plants.
Mayerhofer, R., Koncz-Kalman, Z., Nawrath, C., Bakkeren, G., Crameri, A., Angelis, K., Redei, G. P., Schell, J., Hohn, B., & Koncz, C. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07999.x · Cited by 318
Cited by
Okushima, Y., Overvoorde, P. J., Arima, K., Alonso, J. M., Chan, A., Chang, C., Ecker, J. R., Hughes, B., Lui, A., Nguyen, D., et al. (2005) · The Plant Cell · doi:10.1105/tpc.104.028316 · Cited by 1,122
Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens
Zheng, Z., Qamar, S. A., Chen, Z., & Mengiste, T. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02901.x · Cited by 988
FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis
Imaizumi, T., Tran, H. G., Swartz, T. E., Briggs, W. R., & Kay, S. A. (2003) · Nature · doi:10.1038/nature02090 · Cited by 593
Autophagic Nutrient Recycling in Arabidopsis Directed by the ATG8 and ATG12 Conjugation Pathways
Thompson, A. R., Doelling, J. H., Suttangkakul, A., & Vierstra, R. D. (2005) · Plant Physiology · doi:10.1104/pp.105.060673 · Cited by 574
Hibara, K.-I., Karim, M. R., Takada, S., Taoka, K.-I., Furutani, M., Aida, M., & Tasaka, M. (2006) · The Plant Cell · doi:10.1105/tpc.106.045716 · Cited by 387
Metadata
- Authors
- Sessions, A., Burke, E., Presting, G., Aux, G., McElver, J., Patton, D., Dietrich, B., Ho, P., Bacwaden, J., Ko, C., Clarke, J. D., Cotton, D., Bullis, D., Snell, J., Miguel, T., Hutchison, D., Kimmerly, B., Mitzel, T., Katagiri, F., Glazebrook, J., Law, M., & Goff, S. A.
- Venue
- The Plant Cell
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-11-20
- Volume
- 14
- Issue
- 12
- Pages
- 2985-2994
- ISSN
- 1040-4651 (ISSN-L)
- 1532-298X
- OpenAlex
- W2143142471
- Cited by
- 961
- References
- 23
- FWCI
- 51.52
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A High-Throughput Arabidopsis Reverse Genetics System. https://app.cochat.ai/papers/10.1105/tpc.004630
- The paper
- Sessions, A., Burke, E., Presting, G., Aux, G., McElver, J., Patton, D., Dietrich, B., Ho, P., Bacwaden, J., Ko, C., Clarke, J. D., Cotton, D., Bullis, D., Snell, J., Miguel, T., Hutchison, D., Kimmerly, B., Mitzel, T., Katagiri, F., . . . Goff, S. A. (2002). A High-Throughput Arabidopsis Reverse Genetics System. The Plant Cell. https://doi.org/10.1105/tpc.004630
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
