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Cited by 31

The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.

Erbb4 regulates the oocyte microenvironment during folliculogenesis

Veikkolainen, Ville, Ali, Nsrein, Doroszko, Milena, Kiviniemi, Antti, Miinalainen, Ilkka, Ohlsson, Claes, Poutanen, Matti, Rahman, Nafis, Elenius, Klaus, Vainio, Seppo J, Naillat, Florence·2020·Human Molecular Genetics
DOI 10.1093/hmg/ddaa161
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Abstract

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders leading to infertility in women affecting reproductive, endocrine and metabolic systems. Recent genomewide association studies on PCOS cohorts revealed a single nucleotide polymorphism (SNP) in the ERBB4 receptor tyrosine kinase 4 gene, but its role in ovary development or during folliculogenesis remains poorly understood. Since no genetic animal models mimicking all PCOS reproductive features are available, we conditionally deleted Erbb4 in murine granulosa cells (GCs) under the control of Amh promoter. While we have demonstrated that Erbb4 deletion displayed aberrant ovarian function by affecting the reproductive function (asynchronous oestrous cycle leading to few ovulations and subfertility) and metabolic function (obesity), their ovaries also present severe structural and functional abnormalities (impaired oocyte development). Hormone analysis revealed an up-regulation of serum luteinizing hormone, hyperandrogenism, increased production of ovarian and circulating anti-Müllerian hormone. Our data implicate that Erbb4 deletion in GCs leads to defective intercellular junctions between the GCs and oocytes, causing changes in the expression of genes regulating the local microenvironment of the follicles. In vitro culture assays reducing the level of Erbb4 via shRNAs confirm that Erbb4 is essential for regulating Amh level. In conclusion, our results indicate a functional role for Erbb4 in the ovary, especially during folliculogenesis and its reduced expression plays an important role in reproductive pathophysiology, such as PCOS development.

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Cites

Research works printed but not linked: 2

  1. Sato, 2018, Health and Medicine—Medical Genetics; Researchers at Tokushima University Target Medical Genetics (Genome-Wide Association Study Identifies ERBB4 on 2q34 as a Novel Locus Associated with Sperm Motility in Japanese Men)
  2. Marshall, 2012, All women with PCOS should be treated for insulin resistance, 18

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Metadata

Authors
Veikkolainen, V., Ali, N., Doroszko, M., Kiviniemi, A., Miinalainen, I., Ohlsson, C., Poutanen, M., Rahman, N., Elenius, K., Vainio, S. J., & Naillat, F.
Affiliations
  • Institute of Biomedicine and MediCity Research Laboratory, University of Turku, FI-20520 Turku, Finland
  • Organogenesis Laboratory, Department of Medical Biochemistry and Molecular Biology, Biocenter Oulu, University of Oulu, FI-90014 Oulu, Finland
  • Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, FI-20520 Turku, Finland
  • Electron Microscopy Unit, Biocenter Oulu, University of Oulu, FI-90220 Oulu, Finland
  • Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden
  • Department of Immunology Genetics and Pathology, Section for Neuro-oncology, Uppsala University, 752 36 Uppsala, Sweden
Venue
Human Molecular Genetics
Publisher
Oxford University Press (OUP)
Published
2020-07-27
Volume
29
Issue
17
Pages
2813-2830
ISSN
  • 0964-6906
  • 1460-2083
DOI
10.1093/hmg/ddaa161
Cited by
31
References
60
Language
en
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Funders
  • University of Oulu Profi (311934)
  • Academy of Finland (206038, 121647, 250900, 260056, 251314)
  • Tekes BioRealHealth (24302443)

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