The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Dual effects of superovulation: loss of maternal and paternal imprinted methylation in a dose-dependent manner
Abstract
Superovulation or ovarian stimulation is currently an indispensable assisted reproductive technology (ART) for human subfertility/infertility treatment. Recently, increased frequencies of imprinting disorders have been correlated with ARTs. Significantly, for Angelman and Beckwith-Wiedemann Syndromes, patients have been identified where ovarian stimulation was the only procedure used by the couple undergoing ART. In many cases, increased risk of genomic imprinting disorders has been attributed to superovulation in combination with inherent subfertility. To distinguish between these contributing factors, carefully controlled experiments are required on spontaneously ovulated, in vivo-fertilized oocytes and their induced-ovulated counterparts, thereby minimizing effects of in vitro manipulations. To this end, effects of superovulation on genomic imprinting were evaluated in a mouse model, where subfertility is not a confounding issue. This work represents the first comprehensive examination of the overall effects of superovulation on imprinted DNA methylation for four imprinted genes in individual blastocyst stage embryos. We demonstrate that superovulation perturbed genomic imprinting of both maternally and paternally expressed genes; loss of Snrpn, Peg3 and Kcnq1ot1 and gain of H19 imprinted methylation were observed. This perturbation was dose-dependent, with aberrant imprinted methylation more frequent at the high hormone dosage. Superovulation is thought to primarily affect oocyte development; thus, effects were expected to be limited to maternal alleles. Our study revealed that maternal as well as paternal H19 methylation was perturbed by superovulation. We postulate that superovulation has dual effects during oogenesis, disrupting acquisition of imprints in growing oocytes, as well as maternal-effect gene products subsequently required for imprint maintenance during pre-implantation development.
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Cites
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Intracytoplasmic Sperm Injection May Increase the Risk of Imprinting Defects
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Beckwith-Wiedemann syndrome and assisted reproduction technology (ART): Table 1
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Cited by
The health of children conceived by ART: ‘the chicken or the egg?’
Berntsen, S., Söderström-Anttila, V., Wennerholm, U.-B., Laivuori, H., Loft, A., Oldereid, N. B., Romundstad, L. B., Bergh, C., & Pinborg, A. (2019) · Human Reproduction Update · doi:10.1093/humupd/dmz001 · Cited by 451
Nelissen, E. C. M., van Montfoort, A. P. A., Dumoulin, J. C. M., & Evers, J. L. H. (2011) · Human Reproduction Update · doi:10.1093/humupd/dmq052 · Cited by 384
Steegers-Theunissen, R. P. M., Twigt, J., Pestinger, V., & Sinclair, K. D. (2013) · Human Reproduction Update · doi:10.1093/humupd/dmt041 · Cited by 378
Albuz, F. K., Sasseville, M., Lane, M., Armstrong, D. T., Thompson, J. G., & Gilchrist, R. B. (2010) · Human Reproduction · doi:10.1093/humrep/deq246 · Cited by 284
Haavaldsen, C., Tanbo, T., & Eskild, A. (2012) · Human Reproduction · doi:10.1093/humrep/der428 · Cited by 120
Metadata
- Authors
- Market-Velker, B. A., Zhang, L., Magri, L. S., Bonvissuto, A. C., & Mann, M. R. W.
- Venue
- Human Molecular Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-10-04
- Volume
- 19
- Issue
- 1
- Pages
- 36-51
- ISSN
- 0964-6906 (ISSN-L)
- 1460-2083
- OpenAlex
- W2130708302
- Cited by
- 332
- References
- 57
- FWCI
- 15.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Market-Velker, B. A., Zhang, L., Magri, L. S., Bonvissuto, A. C., & Mann, M. R. W. (2010). Dual effects of superovulation: loss of maternal and paternal imprinted methylation in a dose-dependent manner. Human Molecular Genetics. https://doi.org/10.1093/hmg/ddp465
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