The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence
Abstract
It has been suggested that mitochondrial DNA defects that effect metabolic capacity may be a proximal cause of failures in oocyte maturation, fertilization, or early embryonic development. Here, the distribution of mitochondria was examined by scanning laser confocal microscopy in living human pronuclear oocytes and cleavage stage embryos, followed either by measurements of the net ATP content of individual blastomeres or anti-tubulin immunofluorescence to determine the relationship between mitochondrial distribution and microtubular organization. The results indicate that specific patterns of perinuclear mitochondrial aggregation and microtubular organization are related, and that asymmetrical mitochondrial distributions at the pronuclear stage can result in some proportion of blastomeres with reduced mitochondrial inheritance and diminished ATP generating capacity. While the inability to divide appears to be a development consequence for an affected blastomere, for the embryo, reduced competence may occur during cleavage if several blastomeres inherit a mitochondrial complement inadequate to support normal cellular functions. The findings provide a possible epigenetic explanation for the variable developmental ability expressed within cohorts of morphologically normal early cleavage stage human embryos obtained by in-vitro fertilization.
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Cites
Van Blerkom, J., Davis, P. W., & Lee, J. (1995) · Human Reproduction · doi:10.1093/oxfordjournals.humrep.a135954 · Cited by 670
Self-citationLong-term two-photon fluorescence imaging of mammalian embryos without compromising viability
Squirrell, J. M., Wokosin, D. L., White, J. G., & Bavister, B. D. (1999) · Nature Biotechnology · doi:10.1038/11698 · Cited by 538
Van Blerkom, J., Antczak, M., & Schrader, R. (1997) · Human Reproduction · doi:10.1093/humrep/12.5.1047 · Cited by 526
Self-citationMOLECULAR GENETIC ASPECTS OF HUMAN MITOCHONDRIAL DISORDERS
Larsson, N.-G., & Clayton, D. A. (1995) · Annual Review of Genetics · doi:10.1146/annurev.ge.29.120195.001055 · Cited by 493
Payne, D., Flaherty, S. P., Barry, M. F., & Matthews, C. D. (1997) · Human Reproduction · doi:10.1093/humrep/12.3.532 · Cited by 438
The successful use of pronuclear embryo transfers the day following oocyte retrieval
Scott, L. A., & Smith, S. (1998) · Human Reproduction · doi:10.1093/humrep/13.4.1003 · Cited by 394
Mitochondrial respiratory chain disorders I: mitochondrial DNA defects
Leonard, J., & Schapira, A. (2000) · The Lancet · doi:10.1016/s0140-6736(99)05225-3 · Cited by 363
Oxygen consumption and energy metabolism of the early mouse embryo
Houghton, F. D., Thompson, J. G., Kennedy, C. J., & Leese, H. J. (1996) · Molecular Reproduction and Development · doi:10.1002/(sici)1098-2795(199608)44:4<476::aid-mrd7>3.0.co;2-i · Cited by 352
Ooplasmic transfer in mature human oocytes
Cohen, J. (1998) · Molecular Human Reproduction · doi:10.1093/molehr/4.3.269 · Cited by 343
Oxygen uptake and carbohydrate metabolism by in vitro derived bovine embryos
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Cited by
Mitochondrial function in the human oocyte and embryo and their role in developmental competence
Van Blerkom, J. (2011) · Mitochondrion · doi:10.1016/j.mito.2010.09.012 · Cited by 657
Self-citationRamalho-Santos, J., Varum, S., Amaral, S., Mota, P. C., Sousa, A. P., & Amaral, A. (2009) · Human Reproduction Update · doi:10.1093/humupd/dmp016 · Cited by 482
Al-Mehdi, A.-B., Pastukh, V. M., Swiger, B. M., Reed, D. J., Patel, M. R., Bardwell, G. C., Pastukh, V. V., Alexeyev, M. F., & Gillespie, M. N. (2012) · Science Signaling · doi:10.1126/scisignal.2002712 · Cited by 386
Ferreira, E. M., Vireque, A. A., Adona, P. R., Meirelles, F. V., Ferriani, R. A., & Navarro, P. A. A. S. (2009) · Theriogenology · doi:10.1016/j.theriogenology.2008.10.023 · Cited by 355
Richani, D., Dunning, K. R., Thompson, J. G., & Gilchrist, R. B. (2021) · Human Reproduction Update · doi:10.1093/humupd/dmaa043 · Cited by 346
Metadata
- Authors
- Van Blerkom, J., Davis, P., & Alexander, S.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-12
- Volume
- 15
- Issue
- 12
- Pages
- 2621-2633
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2161682861
- Cited by
- 305
- References
- 25
- FWCI
- 13.51
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence. https://app.cochat.ai/papers/10.1093/humrep/15.12.2621
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- Van Blerkom, J., Davis, P., & Alexander, S. (2000). Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence. Human Reproduction. https://doi.org/10.1093/humrep/15.12.2621
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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