Facility Design and Associated Services for the Study of Amphibians
Open accessAbstract
The role of facilities and associated services for amphibians has recently undergone diversification. Amphibians traditionally used as research models adjust well to captivity and thrive with established husbandry techniques. However, it is now necessary to maintain hundreds of novel amphibian species in captive breeding, conservation research, and biomedical research programs. These diverse species have a very wide range of husbandry requirements, and in many cases the ultimate survival of threatened species will depend on captive populations. Two critical factors have emerged in the maintenance of amphibians, stringent quarantine and high-quality water. Because exotic diseases such as chytridiomycosis have devastated both natural and captive populations of amphibians, facilities must provide stringent quarantine. The provision of high-quality water is also essential to maintain amphibian health and condition due to the intimate physiological relationship of amphibians to their aquatic environment. Fortunately, novel technologies backed by recent advances in the scientific knowledge of amphibian biology and disease management are available to overcome these challenges. For example, automation can increase the reliability of quarantine and maintain water quality, with a corresponding decrease in handling and the associated disease-transfer risk. It is essential to build facilities with appropriate nontoxic waterproof materials and to provide quarantined amphibian rooms for each population. Other spaces and services include live feed rooms, quarantine stations, isolation rooms, laboratory space, technical support systems, reliable energy and water supplies, high-quality feed, and security. Good husbandry techniques must include reliable and species-specific management by trained staff members who receive support from the administration. It is possible to improve husbandry techniques for many species by sharing knowledge through common information systems. Overall, good facility design corresponds to the efficient use of space, personnel, energy, materials, and other resources.
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Cites
Duellman, W. E. (1994) · Johns Hopkins University Press eBooks · doi:10.56021/9780801847806 · Cited by 4,517 · see citing works
Boyle, D., Boyle, D., Olsen, V., Morgan, J. A. T., & Hyatt, A. D. (2004) · Diseases of Aquatic Organisms · doi:10.3354/dao060141 · Cited by 1,271 · see citing works
A Field Guide to Reptiles and Amphibians of Eastern and Central North America
Conant, R. (1975) · Cited by 1,230 · see citing works
Tadpoles: the biology of anuran larvae
(2000) · Choice Reviews Online · doi:10.5860/choice.37-4506 · Cited by 916 · see citing works
Water quality in warmwater fish ponds
Boyd, C. E. (1979) · Cited by 841 · see citing works
Environmental Physiology of the Amphibians
Wassersug, R. J., Feder, M. E., & Burggren, W. W. (1993) · Copeia · doi:10.2307/1447109 · Cited by 746 · see citing works
Developments in Biological Standardization
Miller, E. (1993) · Journal of Epidemiology & Community Health · doi:10.1136/jech.47.1.78-b · Cited by 683 · see citing works
Second nature : environmental enrichment for captive animals
Shepherdson, D. J., Mellen, J. D., & Hutchins, M. (1998) · Smithsonian Institution Press eBooks · Cited by 628 · see citing works
Vertebrate endocrinology : fundamentals and biomedical implications
Pang, P. K. T., & Schreibman, M. P. (1986) · Cited by 552 · see citing works
MacLaughlin, J. A., Anderson, R. R., & Holick, M. F. (1982) · Science · doi:10.1126/science.6281884 · Cited by 539 · see citing works
Origin of the Amphibian Chytrid Fungus
Weldon, C., du Preez, L. H., Hyatt, A. D., Müller, R., & Speare, R. (2004) · Emerging infectious diseases · doi:10.3201/eid1012.030804 · Cited by 508 · see citing works
A natural history of amphibians
Stebbins, R. C., & Cohen, N. W. (1996) · Choice Reviews Online · doi:10.5860/choice.33-5107 · Cited by 504 · see citing works
Amphibian species of the world
Duellman, W. E. (1993) · Cited by 457 · see citing works
The UFAW Handbook on the Care and Management of Laboratory Animals.
(1958) · Archives of Ophthalmology · doi:10.1001/archopht.1958.00940081000029 · Cited by 451 · see citing works
Effect of season and temperature on mortality in amphibians due to chytridiomycosis
Berger, L., Speare, R., Hines, H., Marantelli, G., Hyatt, A. D., McDonald, K., Skerratt, L. F., Olsen, V., Clarke, J. M., Gillespie, G. R., et al. (2004) · Australian Veterinary Journal · doi:10.1111/j.1751-0813.2004.tb11137.x · Cited by 436 · see citing works
Amphibian Medicine and Captive Husbandry
Wright, K. M., & Whitaker, B. R. (2001) · Cited by 410 · see citing works
Johnson, M., Berger, L., Philips, L., & Speare, R. (2003) · Diseases of Aquatic Organisms · doi:10.3354/dao057255 · Cited by 304 · see citing works
Nitrogen Pollution: An Assessment of Its Threat to Amphibian Survival
Rouse, J. D., Bishop, C. A., & Struger, J. (1999) · Environmental Health Perspectives · doi:10.1289/ehp.99107799 · Cited by 297 · see citing works
Stickney, R. R. (2015) · Cited by 273 · see citing works
Lucas, J., & Southgate, P. (2012) · Wiley eBooks · doi:10.1002/9781118687932 · Cited by 248 · see citing works
Maniero, G. D., & Carey, C. (1997) · Journal of Comparative Physiology B · doi:10.1007/s003600050072 · Cited by 212 · see citing works
SENSITIVITY TO NITRATE AND NITRITE IN POND-BREEDING AMPHIBIANS FROM THE PACIFIC NORTHWEST, USA
Marco, A., Quilchano, C., & Blaustein, A. R. (1999) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620181225 · Cited by 186 · see citing works
Emerging Pathogen of Wild Amphibians in Frogs (Rana catesbeiana) Farmed for International Trade
Mazzoni, R., Cunningham, A. A., Daszak, P., Apolo, A., Perdomo, E., & Speranza, G. (2003) · Emerging infectious diseases · doi:10.3201/eid0908.030030 · Cited by 173 · see citing works
Physiology : Coping with the Environment
Ultsch, G. R. (1999) · Cited by 150 · see citing works
Disappearing jewels : the status of new world amphibians
Boucher, T. M., Chanson, J. S., Cox, N. A., Stuart, S. N., & Young, B. E. (2004) · Cited by 122 · see citing works
Browne, R. K., Seratt, J., Vance, C. K., & Kouba, A. J. (2006) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-4-34 · Cited by 119 · see citing works
Self-citationEnvironmental effects on fish immune mechanisms.
Bly, J. E., Quiniou, S. M. A., & Clem, L. W. (1997) · PubMed · Cited by 115 · see citing works
Influence of Sand in Redds on Survival and Emergence of Brook Trout (Salvelinus fontinalis)
Hausle, D. A., & Coble, D. W. (1976) · Transactions of the American Fisheries Society · doi:10.1577/1548-8659(1976)105 · Cited by 102 · see citing works
Nitrate and Nitrite Toxicity to Salmonoid Fishes
Westin, D. T. (1974) · The Progressive Fish-Culturist · doi:10.1577/1548-8659(1974)36[86:nantts]2.0.co;2 · Cited by 98 · see citing works
The relative susceptibilities of some species of fresh-water fish to poisons?I. Ammonia
BALL, I. (1967) · Water Research · doi:10.1016/0043-1354(67)90019-x · Cited by 95 · see citing works
Successful Recovery of Motility and Fertility of Cryopreserved Cane Toad (Bufo marinus) Sperm
Browne, R. K., Clulow, J., Mahony, M. J., & Clark, A. (1998) · Cryobiology · doi:10.1006/cryo.1998.2129 · Cited by 92 · see citing works
Self-citationEvaporative water loss and skin lipids of anuran amphibians
Withers, P. C., Hillman, S. S., & Drewes, R. C. (1984) · Journal of Experimental Zoology · doi:10.1002/jez.1402320103 · Cited by 86 · see citing works
DIRECT EFFECT OF AMMONIA ON THREE SPECIES OF NORTH AMERICAN ANURAN AMPHIBIANS
Jofré, M. B., & Karasov, W. H. (1999) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620180829 · Cited by 85 · see citing works
Acute Toxicity of Nitrite to Rainbow Trout (Salmo gairdneri)
Russo, R. C., Smith, C. E., & Thurston, R. V. (1974) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f74-208 · Cited by 79 · see citing works
Recommendations for the Care of Amphibians and Reptiles in Academic Institutions
Pough, F. H. (1991) · ILAR Journal · doi:10.1093/ilar.33.4.s1 · Cited by 73 · see citing works
Copper toxicity and copper–zinc interactions in amphibian embryos
Herkovits, J., & Helguero, L. A. (1998) · The Science of The Total Environment · doi:10.1016/s0048-9697(98)00184-3 · Cited by 71 · see citing works
The Care and use of amphibians, reptiles, and fish in research
Schaeffer, D., Kleinow, K. M., & Krulisch, L. (1992) · Cited by 71 · see citing works
Carman, E. N., Ferguson, G. W., Gehrmann, W. H., Chen, T. C., & Holick, M. F. (2000) · Copeia · doi:10.1643/0045-8511(2000)2000[0245:poaafu]2.0.co;2 · Cited by 65 · see citing works
Progesterone improves the number and quality of hormone induced Fowler toad (Bufo fowleri) oocytes
Browne, R. K., Li, H., Seratt, J., & Kouba, A. J. (2006) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-4-3 · Cited by 60 · see citing works
Self-citationDisease transmission between and within species, and the implications for disease control
Caley, P. A., & Hone, J. (2004) · Journal of Applied Ecology · doi:10.1111/j.1365-2664.2004.00867.x · Cited by 58 · see citing works
Amphibians: guidelines for the breeding, care, and management of laboratory animals
MCKINNELL, R. G. (1974) · Cited by 56 · see citing works
High density effects on the growth, development and survival of Litoria aurea tadpoles
Browne, R. K., Pomering, M., & Hamer, A. J. (2002) · Aquaculture · doi:10.1016/s0044-8486(02)00205-3 · Cited by 54 · see citing works
Self-citationGrowth of Xenopus laevis under different laboratory rearing conditions.
Hilken, G., Dimigen, J., & Iglauer, F. (1995) · Laboratory Animals · doi:10.1258/002367795780740276 · Cited by 54 · see citing works
The Effects of Low pH and Aluminum on Breeding Success in the Frog Rana temporaria
Beattie, R. C., & Tyler‐Jones, R. (1992) · Journal of Herpetology · doi:10.2307/1565111 · Cited by 53 · see citing works
Belden, L. K., Moore, I. T., Mason, R. T., Wingfield, J. C., & Blaustein, A. R. (2003) · Functional Ecology · doi:10.1046/j.1365-2435.2003.00746.x · Cited by 52 · see citing works
Reproduction and Larval Rearing of Amphibians
Browne, R. K., & Zippel, K. C. (2007) · ILAR Journal · doi:10.1093/ilar.48.3.214 · Cited by 51 · see citing works
Self-citationArthur, J., & Eaton, J. G. (1971) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f71-278 · Cited by 49 · see citing works
Stiffler, D. F. (1993) · Journal of Experimental Biology · doi:10.1242/jeb.184.1.47 · Cited by 48 · see citing works
An Introduction to the Mexican Axolotl (Ambystoma mexicanum)
Gresens, J. M. (2004) · Lab Animal · doi:10.1038/laban1004-41 · Cited by 47 · see citing works
Housing and Husbandry of Xenopus for Oocyte Production
Schultz, T. W., & Dawson, D. A. (2003) · Lab Animal · doi:10.1038/laban0203-34 · Cited by 42 · see citing works
Captive management and conservation of amphibians and reptiles
Murphy, J. B., Adler, K., Collins, J. T., Reptiles, & League, H. (1994) · Cited by 42 · see citing works
THE SUSCEPTIBILITY OF SALMONID FISH TO POISONS UNDER ESTUARINE CONDITIONS. II. AMMONIUM CHLORIDE.
Dw, H., & SHURBEN, D. S. (1965) · PubMed · Cited by 38 · see citing works
Amphibian Distribution with Respect to Pond Water Chemistry near Sudbury, Ontario
Glooschenko, V., Weller, W. F., Smith, P. G. R., Alvo, R., & Archbold, J. H. G. (1992) · Canadian Journal of Fisheries and Aquatic Sciences · doi:10.1139/f92-306 · Cited by 37 · see citing works
Effects of Boron and Nitrate on Hatching Success of Amphibian Eggs
Laposata, M. M., & Dunson, W. A. (1998) · Archives of Environmental Contamination and Toxicology · doi:10.1007/s002449900423 · Cited by 33 · see citing works
Browne, R. K., & Edwards, D. L. (2003) · Journal of Thermal Biology · doi:10.1016/s0306-4565(03)00006-8 · Cited by 32 · see citing works
Self-citationInduced ovulation and egg deposition in the direct developing anuran Eleutherodactylus coqui
Michael, S. F., Buckley, C., Toro, E., Estrada, A. R., & Vincent, S. E. (2004) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-2-6 · Cited by 29 · see citing works
Rodrı́guez-Serna, M., Flores-Nava, A., Olvera-Novoa, M. A., & Carmona-Osalde, C. (1996) · Aquacultural Engineering · doi:10.1016/0144-8609(96)00262-2 · Cited by 25 · see citing works
Latex laboratory-gloves: an unexpected pitfall in amphibian toxicity assays with tadpoles
Gutleb, A. C., Bronkhorst, M., van den Berg, J. H. J., & Murk, A. J. (2001) · Environmental Toxicology and Pharmacology · doi:10.1016/s1382-6689(01)00091-6 · Cited by 23 · see citing works
Murphy, J. B., & Zimmermann, E. (1987) · Copeia · doi:10.2307/1445583 · Cited by 21 · see citing works
Crump, M. L., & Mattison, C. (1989) · Copeia · doi:10.2307/1445637 · Cited by 20 · see citing works
Amphibians as Laboratory Animals
DeNardo, D. F. (1995) · ILAR Journal · doi:10.1093/ilar.37.4.173 · Cited by 17 · see citing works
Amphibians: the world of frogs, toads, salamanders and newts
(2001) · Choice Reviews Online · doi:10.5860/choice.38-3905 · Cited by 17 · see citing works
Toro, E., & Michael, S. F. (2004) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-2-60 · Cited by 16 · see citing works
Pancharatna, K., & Patil, M. M. (1997) · Journal of Herpetology · doi:10.2307/1565338 · Cited by 15 · see citing works
Regulation of calcium and phosphate
Pang, P. K. T., & Schreibman, M. P. (1989) · Academic Press eBooks · Cited by 12 · see citing works
Strimple, P. D. (1996) · Cited by 5 · see citing works
Fraile, B., Paniagua, R., Rodríguez, M. C., & SÁEZ, F. J. (1989) · Copeia · doi:10.2307/1445432 · Cited by 5 · see citing works
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1 of the 68 references couldn’t be matched to a known paper.
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Densmore, C. L., & Green, D. E. (2007) · ILAR Journal · doi:10.1093/ilar.48.3.235 · Cited by 278 · see citing works
Hickman, D. L., Johnson, J., Vemulapalli, T. H., Crisler, J. R., & Shepherd, R. K. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802151-4.00007-4 · Cited by 150 · see citing works
Gyllenhammar, I., Holm, L., Eklund, R., & Berg, C. (2008) · Aquatic Toxicology · doi:10.1016/j.aquatox.2008.06.019 · Cited by 87 · see citing works
Amphibians and conservation breeding programmes: do all threatened amphibians belong on the ark?
Tapley, B., Bradfield, K. S., Michaels, C. J., & Bungard, M. (2015) · Biodiversity and Conservation · doi:10.1007/s10531-015-0966-9 · Cited by 79 · see citing works
Amphibian Biology and Husbandry
Pough, F. H. (2007) · ILAR Journal · doi:10.1093/ilar.48.3.203 · Cited by 74 · see citing works
First line of defence: the role of sloughing in the regulation of cutaneous microbes in frogs
Cramp, R. L., McPhee, R. K., Meyer, E. A., Ohmer, M. E. B., & Franklin, C. E. (2014) · Conservation Physiology · doi:10.1093/conphys/cou012 · Cited by 53 · see citing works
Gratwicke, B., Ross, H., Batista, A., Cháves, G., Crawford, A. J., Elizondo, L., Estrada, A., Evans, M., Garelle, D., Guerrel, J., et al. (2016) · Animal Conservation · doi:10.1111/acv.12249 · Cited by 35 · see citing works
Nutrition Recommendations for some Captive Amphibian Species (Anura and Caudata)
McWilliams, D. A. (2008) · Cited by 19 · see citing works
South Asian amphibians: taxonomy, diversity and conservation status
Molur, S. (2008) · International Zoo Yearbook · doi:10.1111/j.1748-1090.2008.00050.x · Cited by 19 · see citing works
Browne, R. K., Luo, Q., Wang, P., Mansour, N., Kaurova, S. A., Gakhova, E. N., Shishova, N. V., Uteshev, V. K., Kramarova, L. I., Venu, G., et al. (2024) · Animals · doi:10.3390/ani14233395 · Cited by 16 · see citing works
Self-citationManaging the Health of Captive Groups of Reptiles and Amphibians
van Zanten, T. C., & Simpson, S. C. (2021) · Veterinary Clinics of North America Exotic Animal Practice · doi:10.1016/j.cvex.2021.05.005 · Cited by 11 · see citing works
Design and Management of Research Facilities
Lipman, N. S., & Leary, S. L. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-409527-4.00036-5 · Cited by 11 · see citing works
Research-Relevant Background Lesions and Conditions in Common Avian and Aquatic Species
Mangus, L. M., França, M., Shivaprasad, H. L., & Wolf, J. C. (2021) · ILAR Journal · doi:10.1093/ilar/ilab008 · Cited by 10 · see citing works
Parker-Graham, C. A., Clayton, L. A., & Mangus, L. M. (2019) · Veterinary Clinics of North America Exotic Animal Practice · doi:10.1016/j.cvex.2019.09.005 · Cited by 7 · see citing works
A hibernaculum of two Rhacophorid frog species from India
Saikia, B., Laskar, M. A., Debnath, M., Das, H., & Kharkongor, I. J. (2021) · Reptiles & Amphibians · doi:10.17161/randa.v28i3.15809 · Cited by 5 · see citing works
Unintended Consequences of Aquatic Enrichment in Experimental Biology
Blewett, T. A., Ackerly, K. L., Sundin, J., Clark, T. D., Rowsey, L. E., Griffin, R. A., Metz, M., Kuchenmüller, L., Leeuwis, R. H. J., Levet, M., et al. (2025) · Environmental Science & Technology · doi:10.1021/acs.est.4c11276 · Cited by 4 · see citing works
Dysko, R. C., Huerkamp, M. J., Yrjanainen, K. E., Smart, S., Curran, R., Maute, C. J., & Thompson, W. D. (2009) · Elsevier eBooks · doi:10.1016/b978-0-12-369517-8.00032-3 · Cited by 4 · see citing works
An automatic waste‐water disinfection system for an amphibian captive‐breeding and research facility
Robertson, H., Eden, P. A., Gaikhorst, G., MATSON, P. L., Slattery, T., & Vitali, S. D. (2008) · International Zoo Yearbook · doi:10.1111/j.1748-1090.2008.00048.x · Cited by 4 · see citing works
Smith, S. A., & Stoskopf, M. K. (2007) · ILAR Journal · doi:10.1093/ilar.48.3.179 · Cited by 4 · see citing works
Amphibians: biology and captive care
Naguib, M., & Reid, L. (2015) · Companion animal · doi:10.12968/coan.2015.20.12.694 · Cited by 2 · see citing works
Improving Captive Maintenance Techniques for New Zealand Native frogs (Leiopelma spp.)
Shaw, E. J. (2013) · Otago University Research Archive (University of Otago) · Cited by 2 · see citing works
Effects of naturalistic housing conditions on amphibian growth and microbiome in captivity
Touchon, J. C., & Hughey, M. C. (2025) · Animal Microbiome · doi:10.1186/s42523-025-00491-z · Cited by 1 · see citing works
Identification and Validation of Operational Pain Indicators in Anurans
González, S., Caiozzi, A., Cabeza, O., & Cañon–Jones, H. (2025) · Journal of Zoological and Botanical Gardens · doi:10.3390/jzbg6040049 · Cited by 1 · see citing works
Linking Husbandry and Behavior to Enhance Amphibian Reintroduction Success
Linhoff, L. (2018) · doi:10.25148/etd.fidc006549 · Cited by 1 · see citing works
Linhoff, L., Germano, J., & Molinia, F. C. (2022) · CSIRO Publishing eBooks · doi:10.1071/9781486313341.bk07961_ch03
Nonconventional Animal Genetic Resources, Diversity, and Unique Features
Etchu, K. A., Ameur, A. A., Chahbar, M., Meutchieye, F., Njembele, A. N. E., Taboue, G. C. T., & Gaouar, S. B. S. (2025) · Sustainable development goals series · doi:10.1007/978-3-031-92076-9_13
- Authors
- Browne, R. K., Odum, R. A., Herman, T., & Zippel, K.
- Corresponding author
- Browne, R. K.
- ORCID
- Browne, R. K.
- Venue
- ILAR Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-01-01
- Volume
- 48
- Issue
- 3
- Pages
- 188-202
- ISSN
- 1084-2020 (ISSN-L)
- OpenAlex
- W2145840629
- Cited by
- 26
- References
- 68
- Citations by year
- 2025: 4 · 2024: 1 · 2022: 1 · 2021: 3 · 2019: 1 · 2018: 1 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- other-oa
- Locations
- ILAR Journal (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Global and Planetary Change › Amphibian and Reptile Biology
- Health Sciences › Veterinary › Small Animals › Animal Behavior and Welfare Studies
- Physical Sciences › Environmental Science › Management, Monitoring, Policy and Law › Conservation, Ecology, Wildlife Education
- Keywords
- amphibian husbandry, captive breeding, laboratory design, quarantine, water quality, chytridiomycosis, amphibian conservation, automation, disease transmission, live feed
- MeSH terms
- Amphibians, Animals, Animals, Laboratory, Environment, Controlled, Facility Design and Construction, Housing, Animal, Lighting, Quarantine, Ventilation, Water Supply, Medical Laboratory Personnel
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