The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Detection of Cytomegalovirus in Urine from Newborns by Using Polymerase Chain Reaction DNA Amplification
Abstract
Polymerase chain reaction (PCR) amplification was used to detect cytomegalovirus (CMV) in tissue culture and in urine specimens from newborns. Synthetic oligonucleotide primer pairs were used to amplify DNA from the major immediate-early and the late antigen genes of CMV. Amplified products were detected by gel electrophoresis and by dot-blot hybridization with oligonucleotide probes. Using one or both of the primer pairs and associated probes, we found 46 different tissue culture isolates of CMV that were positive; no reaction products were detected when the same primers and probes were used to amplify other herpes family viruses or human genomic DNA. Urine samples from 44 congenitally infected infants were positive when tested with one or both primer pairs and probes. When compared with tissue culture, detection by gel electrophoresis provided a sensitivity of 93%, a specificity of 100%, and a predictive value of a positive result of 100%. Dot-blot analysis raised the sensitivity to 100%. We conclude that PCR amplification may be a valuable tool for diagnosing congenital CMV infection.
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Cites
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Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
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DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear Cells
Ou, C.-Y., Kwok, S., Mitchell, S. W., Mack, D. H., Sninsky, J. J., Krebs, J. W., Feorino, P., Warfield, D., & Schochetman, G. (1988) · Science · doi:10.1126/science.3336784 · Cited by 972
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Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.
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[15] Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method
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Cited by
Eisenach, K. D., Donald Cave, M., Bates, J. H., & Crawford, J. T. (1990) · Journal of Infectious Diseases · doi:10.1093/infdis/161.5.977 · Cited by 748
Metadata
- Authors
- Demmler, G. J., Buffone, G. J., Schimbor, C. M., & May, R. A.
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-12-01
- Volume
- 158
- Issue
- 6
- Pages
- 1177-1184
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2037857358
- Cited by
- 481
- References
- 15
- FWCI
- 29.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Demmler, G. J., Buffone, G. J., Schimbor, C. M., & May, R. A. (1988). Detection of Cytomegalovirus in Urine from Newborns by Using Polymerase Chain Reaction DNA Amplification. Journal of Infectious Diseases. https://doi.org/10.1093/infdis/158.6.1177
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
