Formylated Peptides Are Important Virulence Factors in Staphylococcus aureus Arthritis in Mice
Abstract
BACKGROUND: Staphylococcus aureus is the most common pathogen causing septic arthritis in humans. The affected joints are often rapidly and permanently damaged despite antibiotic treatment, indicating that the elicited host immune response contributes substantially to joint destruction. Bacterial formylated peptides are important chemotactic molecules mediating neutrophil recruitment into infected tissues as an important first step of host defense against invading bacteria. The role of formylated peptides in S. aureus infections has been unknown. METHODS: Mice were intravenously inoculated with wild-type S. aureus strain RN4220 or its isogenic mutant strain (Δfmt) lacking the ability to produce formylated peptides. The development of arthritis was followed clinically and histopathologically. RESULTS: Mice inoculated with the formyl peptide-producing wild-type strain showed a significantly increased frequency and severity of arthritis and subsequent joint destruction as compared with Δfmt mutant strain-inoculated mice. The wild-type S. aureus strain also induced significantly more weight loss than the Δfmt mutant strain. The recruitment of neutrophils into infected kidneys and synovial tissue was significantly higher in mice inoculated with the wild-type strain. CONCLUSIONS: Our data show that formylated peptides function as important virulence factors in S. aureus arthritis, partly by mediating neutrophil recruitment, which contributes substantially to the joint damage.
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In animal models of septic arthritis, bacteria-derived fMet peptides are virulence factors contributing to joint damage via neutrophil-mediated inflammation.
“Notably, data from animal models of septic arthritis have shown that bacteria-derived fMet peptides are virulence factors that contribute to joint damage via neutrophil-mediated inflammation in the joints184.”
From The role of mitochondria in rheumatic diseases (2022) · cites this paper as [184]
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- Authors
- Gjertsson, I., Jonsson, I.-M., Peschel, A., Tarkowski, A., & Lindholm, C.
- Affiliations
- University of Gothenburg (SE)
- Universitätsklinikum Tübingen (DE)
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-11-18
- Volume
- 205
- Issue
- 2
- Pages
- 305-311
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2145659624
- Cited by
- 29
- References
- 20
- Citations by year
- 2024: 2 · 2022: 4 · 2021: 8 · 2019: 1 · 2018: 2 · 2017: 2 · 2015: 4 · 2014: 3 · 2013: 3
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Health Sciences › Medicine › Surgery › Orthopedic Infections and Treatments
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Streptococcal Infections and Treatments
- Keywords
- Staphylococcus aureus, septic arthritis, virulence factors, neutrophil recruitment, chemotaxis, joint destruction, host immune response, synovium, pyelonephritis
- MeSH terms
- Animals, Arthritis, Infectious, Chemotactic Factors, Chemotaxis, Leukocyte, Female, Hindlimb, Kidney, Peroxidase, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, Staphylococcal Infections, Staphylococcus aureus, Synovial Membrane, Weight Loss, Interleukin-6, Hydroxymethyl and Formyl Transferases, Mice
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