The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
High-level IL-12 production by human dendritic cells requires two signals
Abstract
IL-12 is a key cytokine in the development of Th1 responses. IL-12 production by antigen-presenting cells (APC) can be induced by the interaction between CD40 on the APC and CD40 ligand (CD40L) expressed on T cells after activation. Our previous study indicated that in dendritic cells (DC), the only APC that can activate naive T(h) cells efficiently, the mere CD40 engagement is insufficient to induce IL-12 production. The aim of the present study was to dissect the conditions for efficient IL-12 production by DC further. Using populations of naive and memory Th cells, recombinant CD40L, neutralizing and blocking antibodies, and by determining IFN-gamma production and CD40L expression levels, we here show that T cell-induced IL-12 production by DC results from the action of two signals, mediated by CD40L and IFN-gamma, and that the inability of naive T(h) cells to induce IL-12 production resides in their inability to produce IFN-(gamma). Other factors than CD40L and IFN-gamma can provide the required signals for IL-12 production by DC, as either factor could be replaced by lipopolysaccharide (LPS). The two-signal requirement proved unique for the production of IL-12, since either CD40 engagement or LPS was sufficient for the efficient production of tumor necrosis factor-alpha, IL-8 and the p40 subunit of IL-12, and may be considered as a safety mechanism for optimal control of potentially harmful T(h)1 responses.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Sallusto, F., & Lanzavecchia, A. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.179.4.1109 · Cited by 5,129
Development of T H 1 CD4 + T Cells Through IL-12 Produced by Listeria -Induced Macrophages
Hsieh, C.-S., Macatonia, S. E., Tripp, C. S., Wolf, S. F., O'Garra, A., & Murphy, K. M. (1993) · Science · doi:10.1126/science.8097338 · Cited by 3,134
Cella, M., Scheidegger, D., Palmer-Lehmann, K., Lane, P., Lanzavecchia, A., & Alber, G. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.747 · Cited by 1,983
Manetti, R., Parronchi, P., Giudizi, M. G., Piccinni, M. P., Maggi, E., Trinchieri, G., & Romagnani, S. (1993) · The Journal of experimental medicine · doi:10.1084/jem.177.4.1199 · Cited by 1,665
Dendritic cells produce IL-12 and direct the development of Th1 cells from naive CD4+ T cells.
Macatonia, S. E., Hosken, N. A., Litton, M., Vieira, P., Hsieh, C. S., Culpepper, J. A., Wysocka, M., Trinchieri, G., Murphy, K. M., & O'Garra, A. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.154.10.5071 · Cited by 1,524
D'Andrea, A., Aste-Amezaga, M., Valiante, N. M., Ma, X., Kubin, M., & Trinchieri, G. (1993) · The Journal of experimental medicine · doi:10.1084/jem.178.3.1041 · Cited by 1,385
D'Andrea, A., Rengaraju, M., Valiante, N. M., Chehimi, J., Kubin, M., Aste, M., Chan, S. H., Kobayashi, M., Young, D., & Nickbarg, E. (1992) · The Journal of experimental medicine · doi:10.1084/jem.176.5.1387 · Cited by 1,144
Th1 and Th2 CD4+ T cells in the pathogenesis of organ-specific autoimmune diseases
Liblau, R. S., Singer, S. M., & McDevitt, H. O. (1995) · Immunology Today · doi:10.1016/0167-5699(95)80068-9 · Cited by 1,105
Seder, R. A., Gazzinelli, R., Sher, A., & Paul, W. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.21.10188 · Cited by 976
Koch, F., Stanzl, U., Jennewein, P., Janke, K., Heufler, C., Kämpgen, E., Romani, N., & Schuler, G. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.741 · Cited by 927
Cited by
Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion
Castro, F., Cardoso, A. P., Gonçalves, R. M., Serre, K., & Oliveira, M. J. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00847 · Cited by 1,224
Schulz, O., Edwards, A. D., Schito, M., Aliberti, J., Manickasingham, S., Sher, A., & Reis e Sousa, C. (2000) · Immunity · doi:10.1016/s1074-7613(00)00045-5 · Cited by 561
Laouar, Y., Sutterwala, F. S., Gorelik, L., & Flavell, R. A. (2005) · Nature Immunology · doi:10.1038/ni1197 · Cited by 451
Deficient IL-12(p35) Gene Expression by Dendritic Cells Derived from Neonatal Monocytes
Goriely, S., Vincart, B., Stordeur, P., Vekemans, J., Willems, F., Goldman, M., & De Wit, D. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.3.2141 · Cited by 349
Blockade of PD‐L1 (B7‐H1) augments human tumor‐specific T cell responses in vitro
Blank, C., Kuball, J., Voelkl, S., Wiendl, H., Becker, B., Walter, B., Majdic, O., Gajewski, T. F., Theobald, M., Andreesen, R., et al. (2006) · International Journal of Cancer · doi:10.1002/ijc.21775 · Cited by 297
Metadata
- Authors
- Snijders, A.
- Venue
- International Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-11-01
- Volume
- 10
- Issue
- 11
- Pages
- 1593-1598
- ISSN
- 0953-8178 (ISSN-L)
- 1460-2377
- OpenAlex
- W2107954314
- Cited by
- 420
- References
- 35
- FWCI
- 8.36
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: High-level IL-12 production by human dendritic cells requires two signals. https://app.cochat.ai/papers/10.1093/intimm/10.11.1593
- The paper
- Snijders, A. (1998). High-level IL-12 production by human dendritic cells requires two signals. International Immunology. https://doi.org/10.1093/intimm/10.11.1593
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
