The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Deficient IL-12(p35) Gene Expression by Dendritic Cells Derived from Neonatal Monocytes
Open accessAbstract
To gain insight into the defects responsible for impaired Th1 responses in human newborns, we analyzed the production of cytokines by dendritic cells (DC) derived from cord blood monocytes. We observed that neonatal DC generated from adherent cord blood mononuclear cells cultured for 6 days in the presence of IL-4 and GM-CSF show a phenotype similar to adult DC generated from adherent PBMC, although they express lower levels of HLA-DR, CD80, and CD40. Measurement of cytokine levels produced by neonatal DC upon stimulation by LPS, CD40 ligation, or poly(I:C) indicated a selective defect in the synthesis of IL-12. Determination of IL-12(p40) and IL-12(p35) mRNA levels by real-time RT-PCR revealed that IL-12(p35) gene expression is highly repressed in stimulated neonatal DC whereas their IL-12(p40) gene expression is not altered. The addition of rIFN-γ to LPS-stimulated newborn DC restored their expression of IL-12(p35) and their synthesis of IL-12 (p70) up to adult levels. Moreover, we observed that neonatal DC are less efficient than adult DC to induce IFN-γ production by allogenic adult CD4+ T cells. This defect was corrected by the addition of rIL-12. We conclude that neonatal DC are characterized by a severe defect in IL-12(p35) gene expression which is responsible for an impaired ability to elicit IFN-γ production by T cells.
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Cites
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Dendritic cells produce IL-12 and direct the development of Th1 cells from naive CD4+ T cells.
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Cited by
Respiratory Viral Infections in Infants: Causes, Clinical Symptoms, Virology, and Immunology
Tregoning, J. S., & Schwarze, J. (2010) · Clinical Microbiology Reviews · doi:10.1128/cmr.00032-09 · Cited by 747
Basha, S., Surendran, N., & Pichichero, M. (2014) · Expert Review of Clinical Immunology · doi:10.1586/1744666x.2014.942288 · Cited by 513
Newton, S. M., Brent, A. J., Anderson, S., Whittaker, E., & Kampmann, B. (2008) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(08)70182-8 · Cited by 480
Early life: gut microbiota and immune development in infancy
Martin, R., Nauta, A., Ben Amor, K., Knippels, L., Knol, J., & Garssen, J. (2010) · Beneficial Microbes · doi:10.3920/bm2010.0027 · Cited by 338
Wampach, L., Heintz-Buschart, A., Fritz, J. V., Ramiro-Garcia, J., Habier, J., Herold, M., Narayanasamy, S., Kaysen, A., Hogan, A. H., Bindl, L., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07631-x · Cited by 292
Metadata
- Authors
- Goriely, S., Vincart, B., Stordeur, P., Vekemans, J., Willems, F., Goldman, M., & De Wit, D.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-02-01
- Volume
- 166
- Issue
- 3
- Pages
- 2141-2146
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1578914995
- Cited by
- 349
- References
- 24
- FWCI
- 8.03
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Goriely, S., Vincart, B., Stordeur, P., Vekemans, J., Willems, F., Goldman, M., & De Wit, D. (2001). Deficient IL-12(p35) Gene Expression by Dendritic Cells Derived from Neonatal Monocytes. The Journal of Immunology. https://doi.org/10.4049/jimmunol.166.3.2141
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