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Cited by 18Antibiotic Resistance in BacteriaAntimicrobial Resistance in StaphylococcusSalmonella and Campylobacter epidemiology

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Relevance of hot spots in the evolution and transmission of Tn1546 in glycopeptide-resistant Enterococcus faecium (GREF) from broiler origin

Garcia-Migura, L., Hasman, H., Svendsen, C., Jensen, L. B.·2008·Journal of Antimicrobial Chemotherapy
DOI 10.1093/jac/dkn265
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Abstract

OBJECTIVES: Glycopeptide-resistant enterococci are still present within the broiler sector, despite the EU ban of avoparcin more than a decade ago. In the present study, we have developed a rapid method for screening the flanking regions at the integration point of Tn1546 in glycopeptide-resistant Enterococcus faecium isolated from broiler farms. METHODS: Total DNA was digested, ligated and amplified using primers from inside Tn1546. The resulting amplicons were purified and sequenced. Two new primers were designed based on obtained sequences. RESULTS: Two main insertion points have been repeatedly found in isolates from the UK (n = 150). The first insertion point revealed that 25 isolates harboured Tn1546 positioned in a sequence with 96% homology to a streptomycin adenyltransferase gene (AY604739) from a Staphylococcus intermedius plasmid. At this insertion point, a direct repeat (GTCCT) was duplicated as previously described, indicating transposition at the target site. Furthermore, this 'hot spot' was also detected in isolates from Norway (2/8) and Denmark (17/20). The second insertion point detected in 45 isolates from the UK revealed integration into an Inc18-like plasmid, most likely by a process of target site recombination. CONCLUSIONS: The presence of a common insertion point for isolates from different geographical areas could suggest the insertion of Tn1546 by transposition in a plasmid-specific site, followed by genetic rearrangement both inside the transposon and in the flanking regions.

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Crossref · 10.1093/jac/dkn265

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31 of 31 references verified0 citation errors · 0 not checkable

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  • In some eubacteria and archaea, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.

    “In some eubacteria and archaea, such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecium, Lactococcus lactis, and Mycobacterium smegmatis, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.33–36”

    From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [34]

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Metadata

Authors
Garcia-Migura, L., Hasman, H., Svendsen, C., & Jensen, L. B.
Affiliations
Technical University of Denmark (DK)
Corresponding author
Garcia-Migura, L.
ORCID
Venue
Journal of Antimicrobial Chemotherapy
Publisher
Oxford University Press (OUP)
Published
2008-06-27
Volume
62
Issue
4
Pages
681-687
ISSN
  • 0305-7453 (ISSN-L)
  • 1460-2091
DOI
10.1093/jac/dkn265
OpenAlex
W2122786673
Cited by
18
References
31
Citations by year
2023: 1 · 2018: 4 · 2016: 4 · 2013: 2 · 2012: 1
FWCI
0.98
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Antimicrobial Chemotherapy (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
European Commission
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
Keywords
Tn1546 transposon, glycopeptide resistance, Enterococcus faecium, broiler chickens, transposon insertion sites, target site duplication, antimicrobial resistance transmission
MeSH terms
Animals, Anti-Bacterial Agents, Base Sequence, Denmark, DNA Transposable Elements, DNA, Bacterial, Genes, Bacterial, Glycopeptides, United Kingdom, Molecular Sequence Data, Norway, Nucleotidyltransferases, Plasmids, Poultry, Recombination, Genetic, Repetitive Sequences, Nucleic Acid, Enterococcus faecium, Sequence Analysis, DNA, Gene Order, Drug Resistance, Bacterial

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