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Cited by 20Research on Leishmaniasis StudiesLeprosy Research and TreatmentTrypanosoma species research and implications

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Asiaticoside induces tumour-necrosis-factor-α-mediated nitric oxide production to cure experimental visceral leishmaniasis caused by antimony-susceptible and -resistant Leishmania donovani strains

Bhaumik, Siddhartha Kumar, Paul, Joydeep, Naskar, Kshudiram, Karmakar, Subir, De, Tripti·2012·Journal of Antimicrobial Chemotherapy
DOI 10.1093/jac/dkr575
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Abstract

Objectives The aim of this study was to investigate and characterize the efficacy of asiaticoside in an experimental model of visceral leishmaniasis caused by antimony-susceptible (AG83) and -resistant (GE1F8R and K39) Leishmania donovani. Methods The effect of asiaticoside was evaluated by microscopic counting of intracellular amastigotes in cultured macrophages stained with Giemsa. The antileishmanial effect of the compounds was assessed in infected BALB/c mice by estimation of splenic and liver parasite burdens in Leishman Donovan units. Cytokines were measured by real-time PCR and ELISA. Intracellular tumour necrosis factor-α (TNF-α) was measured by fluorescence-activated cell sorting. Nitric oxide was measured by the Griess reaction. Results Besides effectively inhibiting in vitro replication of the parasite within macrophages, asiaticoside treatment resulted in almost complete clearance of the liver and splenic parasite burden when administered at a dose of 5 mg/kg × 10 starting on day +30 of challenge with antimony-susceptible (AG83) and -resistant (GE1F8R and K39) L. donovani. Asiaticoside treatment was associated with a switch in the host from a Th2- to a Th1-type immune response accompanied by the induction of TNF-α-mediated nitric oxide production, all of which are important elements for macrophage function in antileishmanial defence mechanisms. Conclusions These results suggest that oral therapy with asiaticoside shows promising antileishmanial efficacy in animals…

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  • Asiaticoside and madecassoside protect against bacterial, fungal and parasitic infections.

    “protecting against bacterial, fungal and parasitic infections, 10 , 37 , 38”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [38]

  • Asiaticoside and madecassoside play a significant role in improving organ injuries or damage, repressing inflammation and oxidative stress.

    “These triterpene saponins are also confirmed to play a significant role in improving organ injuries or damage, repressing inflammation and oxidative stress, protecting against bacterial, fungal and parasitic infections, 10 , 37 , 38”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [38]

  • Oral administration of asiaticoside is effective in treating parasitic infection of visceral leishmaniasis caused by Leishmania donovani in infected mice models.

    “Oral administration of asiaticoside has proved effective in treating the parasitic infection of visceral leishmaniasis caused by Leishmania donovani in infected mice models.”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [38]

  • Asiaticoside cleared almost all parasitic burdens in liver and spleen and mediated switch in host from Th2- to Th1-type immune response, accompanied by induction of TNF-α-mediated nitric oxide production.

    “Asiaticoside cleared almost all parasitic burdens in the liver and spleen as well as mediated a switch in the host from a Th2‐ to a Th1‐type immune response, accompanied by induction of TNF‐α‐mediated nitric oxide production”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [38]

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Metadata

Authors
Bhaumik, S. K., Paul, J., Naskar, K., Karmakar, S., & De, T.
Affiliations
Indian Institute of Chemical Biology (IN)
ORCID
Venue
Journal of Antimicrobial Chemotherapy
Publisher
Oxford University Press (OUP)
Published
2012-01-18
Volume
67
Issue
4
Pages
910-920
ISSN
  • 0305-7453 (ISSN-L)
  • 1460-2091
DOI
10.1093/jac/dkr575
OpenAlex
W2112176840
Cited by
20
References
0
Citations by year
2024: 1 · 2023: 1 · 2022: 6 · 2021: 4 · 2020: 2 · 2018: 2 · 2016: 2 · 2012: 2
FWCI
0.62
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Antimicrobial Chemotherapy (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
  • Health Sciences › Medicine › Infectious Diseases › Leprosy Research and Treatment
  • Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
Keywords
asiaticoside, visceral leishmaniasis, Leishmania donovani, antimonial resistance, nitric oxide, TNF-α, Th1/Th2 immune response, antileishmanial activity, oral therapy, infection intensity
MeSH terms
Animals, Antimony, Antiprotozoal Agents, Disease Models, Animal, Drug Resistance, Enzyme-Linked Immunosorbent Assay, Female, Leishmania donovani, Leishmaniasis, Visceral, Liver, Macrophages, Mice, Inbred BALB C, Microscopy, Nitric Oxide, Spleen, Triterpenes, Tumor Necrosis Factor-alpha, Polymerase Chain Reaction, Treatment Outcome, Mice

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