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Cited by 266Medicinal Plants and NeuroprotectionNatural product bioactivities and synthesisSkin Protection and Aging

Triterpene Composition and Bioactivities of Centella asiatica

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Hashim, Puziah, Sidek, Hamidah, Helan, Mohd Helme M., Sabery, Aidawati, Palanisamy, Uma Devi, Ilham, Mohd·2011·Molecules
DOI 10.3390/molecules16021310PMC PMC6259745
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Abstract

Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC-PAD on an Excil ODS 5 mm (C18) column for the simultaneous determination of asiatic acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside (1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella (84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic activity was observed by the release of glycerol (115.9 µmol/L) at 0.02% concentration. Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. In view of these results, the potential application of Centella in food and pharmaceutical industries is now widely open.

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  • Madecassoside occupies the highest concentration among the triterpene saponins in most C. asiatica extracts.

    “Madecassoside occupies the highest concentration among the triterpene saponins in most C. asiatica extracts. 5”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [5]

    The Results and Discussion section (page unavailable) identifies madecassoside as the most abundant component in the Centella leaf extract studied. It does not establish that this is true of most C. asiatica extracts; it notes that triterpene composition varies with location, environmental conditions and accession. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • The saponins and their aglycones are the most abundant pentacyclic triterpenoids in C. asiatica, with asiaticoside and madecassoside accounting for roughly 8% of the herb dry mass.

    “The saponins and their aglycones are the most abundant pentacyclic triterpenoids in C. asiatica with asiaticoside and madecassoside accounting for roughly 8% of the herb dry mass. 4 , 5”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [5]

    The Results and Discussion section reports that the leaf extract contains more glycosides than aglycones, with madecassoside and asiaticoside the two most abundant triterpenes measured. It gives their concentrations in mg/mL of extract, not as a percentage of herb dry mass, so it does not support the roughly 8% figure. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 34

  1. [1]Brinkhaus B. Lindner M. Schuppan D. Hahn E.G. Chemical, pharmacological and clinical profile of the East Asian medicinal plant Centella asiatica Phytomedicine 2000 7 427 448 10.1016/S0944-7113(00)80065-3 11081995 · DOI · Paper page
  2. [3]Tenni R. Zanaboni G. De Agustini M.P. Rossi A. Bendotti C. Cetta G. Effect of the triterpenoids fraction of Centella asiatica on macromolecules of the connective matrix in human skin fibroblast cultures Italian J. Biochem. 1988 36 69 77 3042688
  3. [5]Maquart F.X. Chasting F. Simeon A. Brembaut P. Gillery P. Wegrosky Y. Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds Eur. J. Dermatol. 1999 9 289 296 10356407
  4. [6]Kartnig T. Clinical applications of Centella asiatica (L) Urb Herbs, Spices and Medicinal Plants Cracker L.E. Simon J.E. Oxyx Press Phoenix, AZ, USA 1988 145 173
  5. [7]Gupta Y.K. Veerendra Kumar M.H. Srivastava A.K. Effect of Centella asiatica on pentylenetetrazole-induced kindling, cognition and oxidative stress in rats Pharmacol. Biochem. Behav. 2003 74 579 585 10.1016/S0091-3057(02)01044-4 12543222 · DOI
  6. [8]Rao S.B. Chetana M. Uma Devi P. Centella asiatica treatment during postnatal periodenhnaces learning and memory in mice Physiol. Behav. 2005 86 449 457 10.1016/j.physbeh.2005.07.019 16214185 · DOI
  7. [9]Chen Y. Han T. Rui Y. Yin M. Qin L. Zheng H. Effects of total triterpenes of Centella asiatica on the depression behavior and concentration of amino acid in forced swimming mice Zhong Yao Cai 2003 26 870 873 15058206
  8. [11]Sampson J.H. Raman A. Karlsen G. Navsaria H. Leigh I.M. In vitro keratinocyte antiproliferation effect of Centella asiatica extract and triterpenoid saponins Phytomedicine 2001 8 230 235 10.1078/0944-7113-00032 11417919 · DOI
  9. [12]Babu T.D. Kuttan G. Padikkala J. Cytotoxic and anti-tumor properties of ceratin taxa of Umbeliferae with special reference to Centella asiatica (L.) Urban J. Ethnopharmcol. 1995 48 53 57 10.1016/0378-8741(95)01284-K 8569247 · DOI
  10. [13]Srivasta R. Shukla Y.N. Kumar S. Chemistry and Pharmacology of Centella asiatica: A review J. Med. Aromat. Plant Sci. 1997 19 1049 1056
  11. [14]Ling A.P.K. Marziah M. Tan S.E. Triterpenoids distribution in whole plant and callus cultures of Centella asiatica accessions In Proceeding of the 16th National Seminar on Natural Products Serdang, Malaysia 24–25 October 2000 UPM Kuala Lumpur, Malaysia 2000 165 168
  12. [15]Ling A.P.K. Triterpene production in Centella asiatica (L.) urban (pegaga) callus and cell suspension cultures PhD thesis Universiti Putra Malaysia 2004
  13. [16]Zheng C.J. Qin L.P. Chemical components of Centella asiatica and their bioactives J. Chin. Integr. Med. 2007 5 348 351 10.3736/jcim20070324 17498500 · DOI
  14. [17]Inamdar P.K. Yeole R.D. Ghogare A.B. de Souza N.J. Determination of biologically active constituents in Centella asiatica J. Chromatogr. A 1996 742 127 130 10.1016/0021-9673(96)00237-3 · DOI
  15. [19]Schaneberg B.T. Mikell J.R. Bedir E. Khan I.A. An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products Pharamazie 2003 58 381 384 12856998
  16. [20]Randriamampionona D. Diallo B. rakotoniriana F. Rabemanantsoa C. Cheuk K. Corbisier A.M. Mahillon J. Ratsimamanga S. Mondher E.J. Comparative analysis of active constituents in Centella asiatica samples from Madagascar: Application for ex situ conservation and clonal propagation Fitoterapia 2007 78 482 489 10.1016/j.fitote.2007.03.016 17560738 · DOI
  17. [22]Rafamantanana M.H. Rozet E. Raoelison G.E. Cheuk K. Ratsimamanga S.U. Hubert P. Quetin-Leclercq J. An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (Apiaceae) J. Chromatogr. B 2009 877 2396 2402 10.1016/j.jchromb.2009.03.018 19349219 · DOI
  18. [23]Maquart F.X. Bellon G. Gillery P. Wegrosky Y. Borel J.P. Stimulation of collagen synthesis in fibroblast cultures by a triterpene extracted from Centella asiatica Connect. Tissue Res. 1990 24 107 120 10.3109/03008209009152427 2354631 · DOI
  19. [24]Bonte F. Dumas M. Chaudagne C. Meybeck A. Asiaticoside and madecassoside activity on human fibroblast type I and III collagen secretion Ann. Pharm. Fr. 1995 53 38 42 7741425
  20. [26]Sunilkumar Parameshwaraiah S. Shivakumar H.G. Evaluation of topical formulations of aqueous extract of Centella asiatica on open wounds in rats Indian J. Exp. Biol. 1998 36 569 572 9731470
  21. [27]Cesarone M.R. Bercaro G. Rulo A. Griffin M. Ricci A. Ippolito E. De Sanctis M.T. Incandela L. Bavera P. Cacchio M. Bucci M. Microcirculatory effects of total triterpenic fraction of Centella asiatica in chronic venous hypertension: measurement by laser Doppler, TcPO2-CO2, and leg volumetry Angiology 2001 52 S45 S48 10.1177/000331970105202S09 11666123 · DOI
  22. [28]Tholon I. Neliat G. Chesne C. Saboureau D. Perrier E. Branka J.E. An in vitro, ex vivo, and in vivo demonstration of the lipolytic effect of slimming liposomes: An unexpected α(2)-adrenergic antagonism J. Cosmet. Sci. 2002 6 209 218 12219247
  23. [29]Hamid A.A. Md Shah Z. Muse R. Mohamed S. Characterisation of antioxidative of Centella asiatica (L) Urban Food Chem. 2002 77 465 469 10.1016/S0308-8146(01)00384-3 · DOI
  24. [30]Mustafa R.A Hamid A.A. Mohamed S. Abu Bakar F. Total phenolic compounds, flavonoids and radical scavenging activity of 21 selected tropical plants J. Food Sci. 2010 75 C28 C35 10.1111/j.1750-3841.2009.01401.x 20492146 · DOI
  25. [31]Subathra M. Shila S. Devi M.A. Panneerselvam C. Emerging role of Centella asiatica in improving age-related neurological antioxidant status Exp. Gerontol. 2005 40 707 715 10.1016/j.exger.2005.06.001 16026958 · DOI
  26. [32]Pittella F. Dutra R.C. Junior D.D. Lopes M.T. Barbosa N. Antioxidant and cytotoxic activites of Centella asiatica (L) Urb Int. J. Mol. Sci. 2009 10 3713 3721 10.3390/ijms10093713 19865514 PMC2769141 · DOI · Paper page
  27. [33]Jayashree G. Kurup Muraleedhara G. Sudarslal S. Jacob V.B. Antioxidant activity of Centella asiatica on lymphoma-bearing mice Fitoterapia 2003 74 431 434 10.1016/S0367-326X(03)00121-7 12837356 · DOI · Paper page
  28. [34]Shukla A. Rasik A.M. Dhawan B.N. Asiaticoside-induced elevation of antioxidant levels in healing wounds Phytother. Res. 1999 13 50 54 10.1002/(SICI)1099-1573(199902)13:1<50::AID-PTR368>3.0.CO;2-V 10189951 · DOI
  29. [36]Chris I.C. Ungureanu M.I. Marton A. Simedrea R. Muresan A. Postescu I. Decca N. Antioxidant effects of a grape seed extract in a rat model of diabetes mellitus Diabetes Vasc. Dis. Res. 2010 6 200 204 20368212
  30. [37]Nutall S.L. Kendall M.J. Bombardelli E. Morazzoni P. An evaluation of the antioxidant activity of a standardized grape seed extract, Leucoselect J. Clin. Pharm. Ther. 1998 23 385 389 10.1046/j.1365-2710.1998.00180.x 9875688 · DOI
  31. [38]Ling L.T. Ammu Kutty Radhakrishnan, Thavamanithevi Subramaniam, Hwee Ming Cheng and Uma D. Palanisamy. Assessment of Antioxidant Capacity and Cytotoxicity of Selected Malaysian Plants Molecules 2010 15 2139 2151 10.3390/molecules15042139 20428033 PMC6257288 · DOI
  32. [39]Scalia S. Mezzena M. Photostabilization effect of quuercetin on the UV filter combination, butyl methoxydibenzoylmethane-octyl methoxycinnamate Photochem. Photobiol. 2010 86 273 278 10.1111/j.1751-1097.2009.00655.x 19947972 · DOI
  33. [40]Choi S. Park Y.I. Lee S.K. Kim J.E. Chung M.H. Aloesin inhibits hyperpigmentation induced by UV radiation Clin. Exp. Dermatol. 2002 27 513 515 10.1046/j.1365-2230.2002.01120.x 12372097 · DOI
  34. [43]Lee S.E. Hwang H.J. Ha J.S. Screening of medicinal plant extracts for antioxidant activity Life Sci. 2003 73 167 179 10.1016/S0024-3205(03)00259-5 12738032 · DOI

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Authors
Hashim, P., Sidek, H., Helan, M. H. M., Sabery, A., Palanisamy, U. D., & Ilham, M.
Affiliations
  • Universiti Putra Malaysia (MY)
  • Sirim Berhad (MY)
  • Monash University Malaysia (MY)
  • Forest Research Institute Malaysia (MY)
Corresponding author
Hashim, Puziah
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2011-01-28
Volume
16
Issue
2
Pages
1310-1322
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules16021310
PMC
PMC6259745
OpenAlex
W1976505302
Cited by
266
References
2
Citations by year
2026: 11 · 2025: 27 · 2024: 16 · 2023: 22 · 2022: 15 · 2021: 35 · 2020: 34 · 2019: 13 · 2018: 21 · 2017: 9 · 2016: 17 · 2015: 20 · 2014: 10 · 2013: 5 · 2012: 11
FWCI
22.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Kementerian Sains, Teknologi dan Inovasi
  • Universiti Putra Malaysia
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Keywords
Centella asiatica, triterpenoids, asiatic acid, madecassic acid, asiaticoside, madecassoside, collagen synthesis, antioxidant activity, lipase activity, UV protection, glycerol release, grape seed extract, vitamin C
MeSH terms
Central Nervous System Stimulants, Animals, Antioxidants, Caffeine, Cells, Cultured, Chromatography, High Pressure Liquid, Collagen, Fibroblasts, Humans, Plant Extracts, Triterpenes, Molecular Structure, Adipocytes, Plant Leaves, Centella

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