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Cited by 88Probiotics and Fermented FoodsGut microbiota and healthInfant Nutrition and Health

Differentiation of Ingested and Endogenous Bifidobacteria by DNA Fingerprinting Demonstrates the Survival of an Unmodified Strain in the Gastrointestinal Tract of Humans , , ,

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Kullen, Martin J., Amann, Mary M., O'Shaughnessy, Matthew J., O'Sullivan, Daniel J., Busta, Francis F., Brady, Linda J.·1997·The Journal of Nutrition
DOI 10.1093/jn/127.1.89
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Abstract

Consumption of bifidobacteria as a dietary adjunct has received considerable attention for its possible role in the maintenance of gastrointestinal health. However, speculation exists about these presumed health benefits because of an inability to assess the fate and mechanism of action of ingested bifidobacteria. Thus, our objective was to examine the fate of ingested bifidobacteria through the gastrointestinal tract. Variations in the highly conserved 16S ribosomal DNA (rDNA) of bifidobacteria from six male subjects (18 to 35 y old) were assessed by restriction fragment length polymorphism (RFLP) analysis. During the 16-d study, 10(10) colony-forming units (CFU) of a commercially available bifidobacteria were delivered to subjects in fluid milk for each of 8 d. During the remaining 8 d, subjects consumed milk without bifidobacteria. Feces were collected at 4-d intervals and plated on selective media. For each subject, 10-15 colonies were randomly selected and used as template for PCR-amplification of 16S rDNA. 16S rDNA was restriction digested and resolved by electrophoresis. The 16S rDNA-RFLP of the ingested bifidobacteria was unique compared with bifidobacteria found in subjects prior to the feeding study. When subjects consumed bifidobacteria, a 16S rDNA-RFLP identical to that of the ingested bifidobacteria was observed in feces. The concentration of the ingested bifidobacteria in feces increased to 67.2 +/- 8.5% (mean +/- SEM) of total bifidobacteria. After feeding stopped, the ingested bifidobacteria diminished and became undetectable. Using this molecular approach to monitor ingested bifidobacteria, we demonstrate the kinetics of passage of this organism through the gastrointestinal tract of healthy humans.

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  • With interruption of probiotic intake, there is a reduction in the microorganism administered in the colon, and with 8 days of supplementation discontinuation, the probiotic is no longer detectable in the gut.

    “With the interruption of probiotic intake, there is a reduction in the microorganism administered in the colon, and with 8 days of supplementation discontinuation, the probiotic is no longer detectable in the gut [236].”

    From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [236]

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Authors
Kullen, M. J., Amann, M. M., O'Shaughnessy, M. J., O'Sullivan, D. J., Busta, F. F., & Brady, L. J.
Affiliations
University of Minnesota (US)
Corresponding author
Kullen, Martin J.
Venue
The Journal of Nutrition
Publisher
Elsevier BV
Published
1997-01
Volume
127
Issue
1
Pages
89-94
ISSN
0022-3166 (ISSN-L)
DOI
10.1093/jn/127.1.89
OpenAlex
W2125687014
Cited by
88
References
26
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2013: 2 · 2012: 8
FWCI
11.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
Bifidobacterium, probiotics, gastrointestinal tract colonization, 16S rRNA gene, restriction fragment length polymorphism, milk supplementation, fecal microbiota
MeSH terms
Administration, Oral, Adolescent, Adult, Bifidobacterium, Digestive System, Humans, Male, Polymorphism, Restriction Fragment Length, Polymerase Chain Reaction, DNA Fingerprinting

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