Gastric colonization of Candida albicans differs in mice fed commercial and purified diets.
Open accessAbstract
It has been difficult to produce persistent colonization by Candida albicans in the gastrointestinal tract of adult mice without the use of antibiotics and immunosuppressants. We hypothesized that diet influences the colonization of C. albicans and tested the hypothesis. BALB/c mice fed either a commercial rodent diet or a nutritionally adequate mixture of purified ingredients were inoculated i.g. with C. albicans (5 x 10(7) cells). Gastrointestinal colonization was examined by fecal culture, tissue culture, and histology. Mice fed the purified diet had a high fecal recovery of C. albicans [5-6log(10) colony forming units (cfu)/g feces] throughout the experimental period (6 wk), and the major site of colonization was the stomach. C. albicans was undetectable in the feces of mice fed the commercial diet 2 wk after inoculation. Immunosuppressants induced systemic dissemination of C. albicans in mice fed the purified diet. The number of lactobacilli and the concentration of organic acids in the stomach were significantly lower in mice fed the purified diet than in those fed the commercial diet. In vitro culture experiments revealed that acetic and lactic acids suppressed the growth of C. albicans. These results suggest that a reduction in lactobacilli in the stomach of mice fed the purified diet contributed to sustained gastric candidiasis. We therefore propose a novel model of sustained gastric candidiasis by a single i.g. inoculation of C. albicans in healthy adult mice fed a purified diet.
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Acetate is a weak organic acid present in the intestine and can be produced by intestinal microbiota.
“Other weak organic acids besides lactate are present in the intestine, namely acetate, which can be produced by intestinal microbiota (Yamaguchi et al., 2005).”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Yamaguchi et al., 2005
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“albicans colonization (34), or a purified diet (PD) that does not require antibiotics (35).”
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- Authors
- Yamaguchi, N., Sonoyama, K., Kikuchi, H., Nagura, T., Aritsuka, T., & Kawabata, J.
- Affiliations
- Hokkaido University (JP)
- Corresponding author
- Sonoyama, Kei
- ORCID
- Sonoyama, Kei
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2005-01
- Volume
- 135
- Issue
- 1
- Pages
- 109-115
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2115414596
- Cited by
- 69
- References
- 38
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 8 · 2023: 1 · 2022: 5 · 2021: 3 · 2020: 1 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 8 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- Candida albicans, gastric colonization, gastric candidiasis, diet, purified diet, lactobacilli, organic acids, acetic acid, lactic acid, immunosuppression, systemic candidiasis, mouse model, fecal recovery
- MeSH terms
- Animals, Animal Feed, Antibodies, Fungal, Candida albicans, Carboxylic Acids, Gastric Mucosa, Gastrointestinal Contents, Male, Mice, Inbred BALB C, Stomach, Mice
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