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Growth of Multicell Spheroids in Tissue Culture as a Model of Nodular Carcinomas 2
Abstract
Chinese hamster V79 lung cells grown in suspension in tissue culture form multicell spheroids morphologically resembling the nodules in syngeneic transplants of the C3H mammary carcinoma in mice and a number of carcinomas in patients. The growth curve of the cell spheroids is similar to that of the transplants in the mouse. On microscopic examination they develop an outer zone containing many cells in division, an intermediate zone which is poorly nourished and oxygenated and contains few cells in mitosis, and a central zone of necrosis. The spheroids can be broken up by trypsinization and the single cells assayed for colony formation. They provide a useful in vitro model for assessment of effects of nutrition and oxygenation on growth and for determination of growth fraction after treatment with drugs or radiation.
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Cited by
The third dimension bridges the gap between cell culture and live tissue
Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817
Cell and environment interactions in tumor microregions: the multicell spheroid model.
Sutherland, R. M. (1988) · Science · doi:10.1126/science.2451290 · Cited by 1,803
Self-citationIn vitro cell migration and invasion assays
Kramer, N., Walzl, A., Unger, C., Rosner, M., Krupitza, G., Hengstschläger, M., & Dolznig, H. (2013) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2012.08.001 · Cited by 1,170
Spheroid culture as a tool for creating 3D complex tissues
Fennema, E., Rivron, N., Rouwkema, J., van Blitterswijk, C., & de Boer, J. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2012.12.003 · Cited by 1,054
Vinci, M., Gowan, S., Boxall, F., Patterson, L., Zimmermann, M., Court, W., Lomas, C., Mendiola, M., Hardisson, D., & Eccles, S. A. (2012) · BMC Biology · doi:10.1186/1741-7007-10-29 · Cited by 1,024
Metadata
- Venue
- JNCI: Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1971-01
- Volume
- 46
- Issue
- 1
- Pages
- 113-20
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W2221584143
- Cited by
- 585
- References
- 0
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Growth of Multicell Spheroids in Tissue Culture as a Model of Nodular Carcinomas 2. (1971). JNCI: Journal of the National Cancer Institute. https://doi.org/10.1093/jnci/46.1.113
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
