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Cited by 13Effects of Radiation ExposureImmunotoxicology and immune responsesSpaceflight effects on biology

Changes in the distribution and function of leukocytes after whole body iron ion irradiation

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Gridley, Daila S., Pecaut, Michael J.·2016·Journal of Radiation Research
DOI 10.1093/jrr/rrw051PMC PMC5045078
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Abstract

High-energy particle radiation could have a considerable impact on health during space missions. This study evaluated C57BL/6 mice on Day 40 after total-body 56 Fe 26+ irradiation at 0, 1, 2 and 3 gray (Gy). Radiation consistently increased thymus mass (one-way ANOVA: P < 0.005); spleen, liver and lung masses were similar among all groups. In the blood, there was no radiation effect on the white blood cell (WBC) count or major leukocyte types. However, the red blood cell count, hemoglobin, hematocrit and the CD8+ T cytotoxic (Tc) cell count and percentage all decreased, while both the CD4:CD8 (Th:Tc) cell ratio and spontaneous blastogenesis increased, in one or more irradiated groups compared with unirradiated controls ( P < 0.05 vs 0 Gy). In contrast, splenic WBC, lymphocyte, B cell and T helper (Th) counts, %B cells and the CD4:CD8 ratio were all significantly elevated, while Tc percentages decreased, in one or more of the irradiated groups compared with controls ( P < 0.05 vs 0 Gy). Although there were trends for minor, radiation-induced increases in %CD11b+ granulocytes in the spleen, cells double-labeled with adhesion markers (CD11b+CD54+, CD11b+CD62E+) were normal. Splenocyte spontaneous blastogenesis and that induced by mitogens (PHA, ConA, LPS) was equivalent to normal. In bone marrow, the percentage of cells expressing stem cell markers, Sca-1 and CD34/Sca-1, were low in one or more of the irradiated groups ( P < 0.05 vs 0 Gy). Collectively, the data indicate that significant immunological abnormalities still exist more than a month after 56 Fe irradiation and that there are differences dependent upon body compartment.

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  • Complete blood count with three-part differential and immunological profiling after exposure to 0, 1, 2, or 3 Gy of total body 56Fe irradiation demonstrated significant immunological abnormalities one month post-exposure including increased hematopoiesis and CD4:CD8 ratio.

    “Complete blood count with three-part differential and immunological profiling after exposure to 0, 1, 2, or 3 Gy of total body 56 Fe irradiation demonstrated significant immunological abnormalities one month-post-exposure including increased hematopoiesis and CD4:CD8 ratio alongside decreased red blood cell count, hematocrit, hemoglobin, and CD8+ T cells (Gridley and Pecaut, 2016).”

    From Medical countermeasures for the hematopoietic-subsyndrome of acute radiation syndrome in space (2022) · cites this paper as Gridley and Pecaut, 2016

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  • Complete blood count with three-part differential and immunological profiling after exposure to 0, 1, 2, or 3 Gy of total body 56Fe irradiation demonstrated decreased red blood cell count, hematocrit, hemoglobin, and CD8+ T cells one month post-exposure.

    “Complete blood count with three-part differential and immunological profiling after exposure to 0, 1, 2, or 3 Gy of total body 56 Fe irradiation demonstrated significant immunological abnormalities one month-post-exposure including increased hematopoiesis and CD4:CD8 ratio alongside decreased red blood cell count, hematocrit, hemoglobin, and CD8+ T cells (Gridley and Pecaut, 2016).”

    From Medical countermeasures for the hematopoietic-subsyndrome of acute radiation syndrome in space (2022) · cites this paper as Gridley and Pecaut, 2016

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Cited by

Sorted by citations

Authors
Gridley, D. S., & Pecaut, M. J.
Affiliations
Loma Linda University (US)
Corresponding author
Pecaut, Michael J.
ORCID
Pecaut, Michael J.
Venue
Journal of Radiation Research
Publisher
Oxford University Press (OUP)
Published
2016-09-01
Volume
57
Issue
5
Pages
477-491
ISSN
  • 0449-3060 (ISSN-L)
  • 1349-9157
DOI
10.1093/jrr/rrw051
PMC
PMC5045078
OpenAlex
W2463773653
Cited by
13
References
5
Citations by year
2024: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2
FWCI
1.45
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Radiation Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • National Aeronautics and Space Administration (NCC9-79)
  • Department of Radiation Medicine of the Loma Linda University Medical Center
  • Department of Basic Sciences at Loma Linda University
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
  • Health Sciences › Medicine › Physiology › Spaceflight effects on biology
Keywords
iron ion irradiation, 56Fe, high-energy particle radiation, space radiation, leukocyte distribution, CD4/CD8 ratio, CD8+ cytotoxic T lymphocytes, T helper cells, B cells, splenocytes, mitogen response, hematopoietic stem cells, Sca-1, granulocytes, radiation-induced immunosuppression, C57BL/6 mice
MeSH terms
Animals, Blood Cell Count, Body Weight, Bone Marrow Cells, Cell Adhesion, Female, Ions, Iron, Leukocytes, Lymphocyte Activation, Mice, Inbred C57BL, Mitogens, Organ Size, Stem Cells, Whole-Body Irradiation, Biomarkers, Lymphocyte Subsets

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