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Cited by 62Inflammatory mediators and NSAID effectsEicosanoids and Hypertension PharmacologyMenstrual Health and Disorders

Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle

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Catalano, R. D., Wilson, M. R., Boddy, S. C., Jabbour, H. N.·2011·Molecular Human Reproduction
DOI 10.1093/molehr/gaq094PMC PMC3037736
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Abstract

Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.

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  • AKR1C3 expression is increased in the secretory phase relative to the proliferative phase, with peak expression in the early- to mid-secretory phase.

    “Assessment of endometrial tissue samples by immunohistochemistry and qRT-PCR of whole-tissue homogenates performed by Catalano et al. demonstrated that AKR1C3 expression is increased in the secretory phase relative to proliferative phase with peak expression reported in the early- to mid-secretory phase [42].”

    From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [42]

    Table II in Results (RNA transcript expression of prostanoid enzymes; page unavailable) reports AKR1C3 RNA expression 5.5-fold higher in early secretory than proliferative endometrium. But early-secretory expression was also significantly higher than mid-secretory expression (2.2-fold), so the reported peak was early secretory, not early-to-mid secretory. The immunohistochemistry Results describe prominent AKR1C3 staining throughout the cycle rather than a secretory-phase staining peak. (LLM, conf=0.89)

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Cited by

Sorted by citations

Authors
Catalano, R. D., Wilson, M. R., Boddy, S. C., & Jabbour, H. N.
Affiliations
The Queen's Medical Research Institute (GB)
Corresponding author
Jabbour, H. N.
Venue
Molecular Human Reproduction
Publisher
Oxford University Press (OUP)
Published
2010-11-26
Volume
17
Issue
3
Pages
182-192
ISSN
  • 1360-9947 (ISSN-L)
  • 1460-2407
DOI
10.1093/molehr/gaq094
PMC
PMC3037736
OpenAlex
W1984195795
Cited by
62
References
0
Citations by year
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FWCI
3.54
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Molecular Human Reproduction (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
Medical Research Council (U.1276.00.004.00002.01)
Topics
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Menstrual Health and Disorders
Keywords
prostanoids, prostanoid receptors, human endometrium, menstrual cycle, prostaglandin F receptor, aldose reductase, CBR1, AKR1C3, 15-hydroxyprostaglandin dehydrogenase, PTGIS, prostaglandin D synthase, prostaglandin E synthase, endometritis, endometrial glandular epithelium, endometrial vasculature
MeSH terms
Aldo-Keto Reductases, Adult, Alcohol Oxidoreductases, Aldehyde Reductase, Endometrium, Female, Humans, Menstrual Cycle, Prostaglandins, Receptors, Prostaglandin, RNA, Messenger

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