The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing
Open accessAbstract
This paper describes a computer method for handling gel reading data produced by the shotgun method of DNA sequencing. The method greatly reduces the time the sequencer needs to spend checking and editing his data and yet it produces a consensus sequence for which the accuracy of determination of every base can be clearly shown. The program can take a batch of new gel readings, screen them against vector sequences removing any that match, and then compare and align all the sequences to produce a final consensus. No information is lost in this process as alignments are achieved by making only insertions and because all the individual gel readings are added to a database from which they can be retrieved and displayed lined up one above the other. This allows the user to check on the alignments achieved by the program and if necessary change them. As each gel reading is added to the database the consensus is automatically updated accordingly and used for the next comparisons. This is a much faster process than comparing each new gel against every individual gel in the database.
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Cites
Needleman, S. B., & Wunsch, C. D. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90057-4 · Cited by 11,288
Cited by
Arriza, J. L., Weinberger, C., Cerelli, G., Glaser, T. M., Handelin, B. L., Housman, D. E., & Evans, R. M. (1987) · Science · doi:10.1126/science.3037703 · Cited by 1,946
Nucleotide sequence of the AIDS virus, LAV
Wain-Hobson, S., Sonigo, P., Danos, O., Cole, S., & Alizon, M. (1985) · Cell · doi:10.1016/0092-8674(85)90303-4 · Cited by 1,202
Robson, K. J. H., Hall, J. R. S., Jennings, M. W., Harris, T. J. R., Marsh, K., Newbold, C. I., Tate, V. E., & Weatherall, D. J. (1988) · Nature · doi:10.1038/335079a0 · Cited by 384
The real cost of sequencing: scaling computation to keep pace with data generation
Muir, P., Li, S., Lou, S., Wang, D., Spakowicz, D. J., Salichos, L., Zhang, J., Weinstock, G. M., Isaacs, F., Rozowsky, J., et al. (2016) · Genome Biology · doi:10.1186/s13059-016-0917-0 · Cited by 367
Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1
McGeoch, D. J., Dolan, A., Donald, S., & Brauer, D. H. K. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.4.1727 · Cited by 332
Metadata
- Authors
- Staden, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1982
- Volume
- 10
- Issue
- 15
- Pages
- 4731-4751
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2152825059
- Cited by
- 735
- References
- 1
- FWCI
- 18.66
- Type
- journal article
- Language
- en
- Open access
- green
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- Staden, R. (1982). Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research. https://doi.org/10.1093/nar/10.15.4731
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
