Effects of DNA looping on pyrimidine dimer formation
Open accessAbstract
We have assessed the effects of DNA curvature on pyrimidine dimer (PD) formation by examining the pattern of PD formation in DNA held in a loop by lambda repressor. The loop region was composed of diverse DNA sequences such that potential PD sites occurred throughout the loop. PD formation in the loop occurred with peaks at approximately 10 base intervals, just 3' of where the bending of the DNA was inferred to be toward the major groove. This relationship between the peaks and the DNA curvature is essentially identical to that observed in the nucleosome. This indicates that DNA curvature is the major source of the periodicity of PD formation in the nucleosome, and supports an earlier model of the conformation of nucleosomal DNA based on PD formation. DNA loops containing diverse sequences should be of general value for assessing the effects of DNA curvature on DNA modification by other agents used to probe DNA-protein interactions and DNA conformation.
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Bending DNA toward the minor groove reduces CPD formation.
“Bending of DNA towards the minor groove reduces CPDs formation [52].”
From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [52]
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“Bending of DNA towards the minor groove reduces CPDs formation [52].”
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- Authors
- Pehrson, J. R., & Cohen, L. H.
- Affiliations
- Fox Chase Cancer Center (US)
- Corresponding author
- Pehrson, John R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1992
- Volume
- 20
- Issue
- 6
- Pages
- 1321-1324
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC312177
- OpenAlex
- W2038445174
- Cited by
- 58
- References
- 25
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2s07rr005539-29, 5r01gm024019-13)
- National Cancer Institute (P30CA006927, CA06927)
- National Institute of General Medical Sciences (GM 24019)
- National Center for Research Resources (RR-05539)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
- Keywords
- DNA looping, DNA curvature, lambda repressor, nucleosome, DNA conformation, protein-DNA interactions
- MeSH terms
- Base Sequence, DNA, DNA-Binding Proteins, Molecular Sequence Data, Nucleic Acid Conformation, Pyrimidine Dimers, Repressor Proteins, Viral Proteins, Viral Regulatory and Accessory Proteins
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