Cited by 98DNA Repair MechanismsDNA and Nucleic Acid ChemistryGenomics and Chromatin Dynamics

UV INDUCED (6‐4) PHOTOPRODUCTS ARE DISTRIBUTED DIFFERENTLY THAN CYCLOBUTANE DIMERS IN NUCLEOSOMES

Gale, James M., Smerdon, Michael J.·1990·Photochemistry and Photobiology

Abstract

Abstract— We have compared the distributions of two stable UV photoproducts in nucleosome core DNA at the single‐nucleotide level using a T4 polymerase‐exonuclease mapping procedure. The distribution of pyrimidine‐pyrimidone (6‐4) dimers was uncovered by reversing the major UV photo‐product, cis‐syn cyclobutane pyrimidine dimer, with E. coli DNA photolyase and photoreactivating light. Whereas the distribution of total UV photoproducts in nucleosome core DNA forms a striking 10.3 base periodic pattern, the distribution of (6‐4) dimers is much more random throughout the nucleosome core domain. Therefore, histone‐DNA interactions in nucleosomes strongly modulate formation of the major class of UV‐induced photoproducts, while having either a constant effect or no effect on (6‐4) dimer formation.

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Crossref · 10.1111/j.1751-1097.1990.tb01732.x

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Authors
Gale, J. M., & Smerdon, M. J.
Affiliations
  • Los Alamos National Laboratory (US)
  • Washington State University (US)
Corresponding author
Smerdon, Michael J.
Venue
Photochemistry and Photobiology
Publisher
Wiley
Published
1990-04
Volume
51
Issue
4
Pages
411-417
ISSN
  • 0031-8655 (ISSN-L)
  • 1751-1097
OpenAlex
W2128799509
Cited by
98
References
39
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 5 · 2015: 7 · 2014: 2 · 2012: 1
FWCI
1.69
Type
journal article
Language
en
Open access
closed
Locations
  • Photochemistry and Photobiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01es003720-03)
  • National Institute of Environmental Health Sciences (ES03720, ES02614)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
6-4 photoproducts, cyclobutane pyrimidine dimers, histone-DNA interactions, DNA photolyase, photoreactivation, DNA damage
MeSH terms
Animals, Cattle, Chromatin, DNA, DNA-Directed DNA Polymerase, Exonucleases, Nucleosomes, Nucleotide Mapping, T-Phages, Thymus Gland, Ultraviolet Rays

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