UV INDUCED (6‐4) PHOTOPRODUCTS ARE DISTRIBUTED DIFFERENTLY THAN CYCLOBUTANE DIMERS IN NUCLEOSOMES
Abstract
Abstract— We have compared the distributions of two stable UV photoproducts in nucleosome core DNA at the single‐nucleotide level using a T4 polymerase‐exonuclease mapping procedure. The distribution of pyrimidine‐pyrimidone (6‐4) dimers was uncovered by reversing the major UV photo‐product, cis‐syn cyclobutane pyrimidine dimer, with E. coli DNA photolyase and photoreactivating light. Whereas the distribution of total UV photoproducts in nucleosome core DNA forms a striking 10.3 base periodic pattern, the distribution of (6‐4) dimers is much more random throughout the nucleosome core domain. Therefore, histone‐DNA interactions in nucleosomes strongly modulate formation of the major class of UV‐induced photoproducts, while having either a constant effect or no effect on (6‐4) dimer formation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Nucleosome positioning modulates photoreactivity and therefore the ratio of 6-4PP to CPD formation.
“Structural parameters have also to be taken into account, such as the nucleosome positioning that modulates the photoreactivity and therefore the ratio formation between 6-4PPs and CPDs (69).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [69]
Cites
Sequence periodicities in chicken nucleosome core DNA
Satchwell, S. C., Drew, H. R., & TRAVERS, A. A. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90452-3 · Cited by 929 · see citing works
Sancar, A., & Sancar, G. B. (1988) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.57.070188.000333 · Cited by 815 · see citing works
Sancar, A., & Rupp, W. D. (1983) · Cell · doi:10.1016/0092-8674(83)90354-9 · Cited by 627 · see citing works
UV-induced mutation hotspots occur at DNA damage hotspots
Brash, D. E., & Haseltine, W. A. (1982) · Nature · doi:10.1038/298189a0 · Cited by 393 · see citing works
Photochemistry and Photobiology of Nucleic Acids
(1976) · Elsevier eBooks · doi:10.1016/b978-0-12-734601-4.x5001-0 · Cited by 380 · see citing works
Structural features of a phased nucleosome core particle.
Simpson, R. T., & Stafford, D. W. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.1.51 · Cited by 324 · see citing works
Biological effects of ultraviolet radiation
Grandolfo, M. (1981) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(81)85017-9 · Cited by 309 · see citing works
Mitchell, D. L., Haipek, C. A., & Clarkson, J. M. (1985) · Mutation Research Letters · doi:10.1016/s0165-7992(85)80018-x · Cited by 277 · see citing works
Gale, J. M., Nissen, K. A., & Smerdon, M. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.19.6644 · Cited by 210 · see citing works
Self-citationMolecular Models for DNA Damaged by Photoreaction
Pearlman, D. A., Holbrook, S. R., Pirkle, D. H., & Kim, S.-H. (1985) · Science · doi:10.1126/science.3975615 · Cited by 183 · see citing works
Unique DNA Repair Properties of a Xeroderma Pigmentosum Revertant
Cleaver, J. E., Cortés, F., Lutze, L. H., Morgan, W. F., Player, A. N., & Mitchell, D. L. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.9.3353 · Cited by 117 · see citing works
Doetsch, P. W., Chan, G. L., & Haseltine, W. A. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.9.3285 · Cited by 105 · see citing works
Hershfield, M. S., & Nossal, N. G. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45153-3 · Cited by 105 · see citing works
The C-C (6-4) UV photoproduct is mutagenic in Escherichia coli.
Glickman, B. W., Schaaper, R. M., Haseltine, W. A., Dunn, R. L., & Brash, D. E. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.18.6945 · Cited by 101 · see citing works
Taylor, J., Garrett, D. S., & Cohrs, M. P. (1988) · Biochemistry · doi:10.1021/bi00419a007 · Cited by 94 · see citing works
Cyclobutane pyrimidine dimers and (6-4) photoproducts block polymerization by DNA polymerase I
Chan, G. L., Doetsch, P. W., & Haseltine, W. A. (1985) · Biochemistry · doi:10.1021/bi00342a006 · Cited by 93 · see citing works
Brash, D. E., Franklin, W. A., Sancar, G. B., Sancar, A., & Haseltine, W. A. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39046-4 · Cited by 92 · see citing works
CHO mutant UV61 removes (6–4) photoproducts but not cyclobutane dimers
Thompson, L. H., Mitchell, D. L., Regan, J. D., Bouffler, S. D., Stewart, S. A., Carrier, W. L., Nairn, R. S., & Johnson, R. T. (1989) · Mutagenesis · doi:10.1093/mutage/4.2.140 · Cited by 90 · see citing works
Gordon, L. K., & Haseltine, W. A. (1982) · Radiation Research · doi:10.2307/3575688 · Cited by 89 · see citing works
Franklin, W. A., Doetsch, P. W., & Haseltine, W. A. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.14.5317 · Cited by 88 · see citing works
Nucleosomal distribution of thymine photodimers following far- and near-ultraviolet irradiation
Niggli, H. J., & Cerutti, P. (1982) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(82)91098-1 · Cited by 87 · see citing works
Photofootprint of nucleosome core DNA in intact chromatin having different structural states
Gale, J. M., & Smerdon, M. J. (1988) · Journal of Molecular Biology · doi:10.1016/0022-2836(88)90054-x · Cited by 80 · see citing works
Self-citationWilliams, J. I., & Friedberg, E. C. (1979) · Biochemistry · doi:10.1021/bi00585a019 · Cited by 79 · see citing works
Binding of Escherichia coli DNA photolyase to UV-irradiated DNA
Sancar, G. B., Smith, F. W., & Sancar, A. (1985) · Biochemistry · doi:10.1021/bi00329a007 · Cited by 77 · see citing works
Jörns, M. S., Sancar, G. B., & Sancar, A. (1985) · Biochemistry · doi:10.1021/bi00329a008 · Cited by 76 · see citing works
The role of the (6−4) photoproduct in ultraviolet light-induced transition mutations in E. coli
Franklin, W. A., & Haseltine, W. A. (1986) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(86)90002-7 · Cited by 74 · see citing works
Mitchell, D. L., & Rosenstein, B. S. (1987) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1987.tb07882.x · Cited by 66 · see citing works
Mitchell, D. L. (1988) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(88)90024-7 · Cited by 60 · see citing works
Myles, G. M., Houten, B. V., & Sancar, A. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.3.1227 · Cited by 50 · see citing works
REPAIR OF CYCLOBUTANE DIMERS AND (6–4) PHOTOPRODUCTS IN ICR 2A FROG CELLS
Mitchell, D. L., Clarkson, J. M., Chao, C. C. ‐., & Rosenstein, B. S. (1986) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1986.tb09539.x · Cited by 50 · see citing works
Mitchell, D. L., Humphrey, R. M., Adair, G. M., Thompson, L. H., & Clarkson, J. M. (1988) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(88)90007-7 · Cited by 48 · see citing works
REDUCED REPAIR OF NON‐DIMER PHOTOPRODUCTS IN A GENE TRANSFECTED INTO XERODERMA PIGMENTOSUM CELLS
Protić‐Sabljić, M., & Kraemer, K. H. (1986) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1986.tb09528.x · Cited by 45 · see citing works
Taylor, J., Garrett, D. S., & Wang, M. J. (1988) · Biopolymers · doi:10.1002/bip.360271004 · Cited by 39 · see citing works
Tang, M.-S., Hrncir, J., Mitchell, D. L., Ross, J. A., & Clarkson, J. (1986) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(86)90095-3 · Cited by 36 · see citing works
Nondimer Damage in Deoxyribonucleic Acid Caused by Ultraviolet Radiation
Rahn, R. O. (1979) · Springer eBooks · doi:10.1007/978-1-4684-3551-1_5 · Cited by 32 · see citing works
Selective visualization of gene structure with ultraviolet light.
Wang, Z., & Becker, M. M. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.3.654 · Cited by 31 · see citing works
Gale, J. M., & Smerdon, M. J. (1988) · Biochemistry · doi:10.1021/bi00419a006 · Cited by 29 · see citing works
Self-citationPeak, M. J., & Peak, J. G. (1986) · Springer eBooks · doi:10.1007/978-1-4615-9462-8_20 · Cited by 28 · see citing works
DNA Excision Repair at the Nucleosome Level of Chromatin
Smerdon, M. J. (1989) · Springer eBooks · doi:10.1007/978-1-4684-1327-4_26 · Cited by 23 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Formation of UV-induced DNA damage contributing to skin cancer development
Cadet, J., & Douki, T. (2018) · Photochemical & Photobiological Sciences · doi:10.1039/c7pp00395a · Cited by 435 · see citing works
Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
DNA repair and the role of chromatin structure
Smerdon, M. J. (1991) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(91)90069-b · Cited by 227 · see citing works
Self-citationLima-Bessa, K. M. D., Armelini, M. G., Chiganças, V., Jacysyn, J. F., Amarante-Mendes, G. P., Sarasin, A., & Menck, C. F. M. (2008) · DNA Repair · doi:10.1016/j.dnarep.2007.11.003 · Cited by 87 · see citing works
Differential DNA lesion formation and repair in heterochromatin and euchromatin
Han, C., Srivastava, A. K., Cui, T., Wang, Q.-E., & Wani, A. A. (2016) · Carcinogenesis · doi:10.1093/carcin/bgv247 · Cited by 58 · see citing works
Effects of DNA looping on pyrimidine dimer formation
Pehrson, J. R., & Cohen, L. H. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.6.1321 · Cited by 58 · see citing works
Suquet, C., & Smerdon, M. J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80444-x · Cited by 53 · see citing works
Self-citationAboussekhra, A., & Thoma, F. (1999) · The EMBO Journal · doi:10.1093/emboj/18.2.433 · Cited by 47 · see citing works
Photobiological Origins of the Field of Genomic Maintenance
Ganesan, A., & Hanawalt, P. (2016) · Photochemistry and Photobiology · doi:10.1111/php.12542 · Cited by 22 · see citing works
- Authors
- Gale, J. M., & Smerdon, M. J.
- Affiliations
- Los Alamos National Laboratory (US)
- Washington State University (US)
- Corresponding author
- Smerdon, Michael J.
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 1990-04
- Volume
- 51
- Issue
- 4
- Pages
- 411-417
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- OpenAlex
- W2128799509
- Cited by
- 98
- References
- 39
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 5 · 2015: 7 · 2014: 2 · 2012: 1
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01es003720-03)
- National Institute of Environmental Health Sciences (ES03720, ES02614)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- 6-4 photoproducts, cyclobutane pyrimidine dimers, histone-DNA interactions, DNA photolyase, photoreactivation, DNA damage
- MeSH terms
- Animals, Cattle, Chromatin, DNA, DNA-Directed DNA Polymerase, Exonucleases, Nucleosomes, Nucleotide Mapping, T-Phages, Thymus Gland, Ultraviolet Rays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review