Association of 2' - 5' oligoribonucleotides
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Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.
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2'-5' linked RNA duplexes have a much lower Tm than natural RNA duplexes.
“It has been known for many years that 2 ' -5 ' linked RNA duplexes have a much lower Tm than natural RNA duplexes [23].”
From The eightfold path to non-enzymatic RNA replication (2012) · cites this paper as [23]
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- Authors
- Kierzek, R., He, L., & Turner, D. H.
- Affiliations
- Institute of Bioorganic Chemistry, Polish Academy of Sciences (PL)
- University of Rochester (US)
- Corresponding author
- Turner, Douglas H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1992
- Volume
- 20
- Issue
- 7
- Pages
- 1685-1690
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC312257
- OpenAlex
- W2034295729
- Cited by
- 79
- References
- 31
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2020: 2 · 2017: 1 · 2016: 2 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (GM 22939)
- National Institute of General Medical Sciences (GM 22939, R01GM022939)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- duplex stability, UV melting, circular dichroism, imino proton NMR, NOE spectroscopy, RNA evolution, RNA secondary structure
- MeSH terms
- Base Sequence, Circular Dichroism, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Oligoribonucleotides, Spectrophotometry, Ultraviolet, Temperature
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