Cited by 79DNA and Nucleic Acid ChemistryRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques

Association of 2' - 5' oligoribonucleotides

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Kierzek, Ryszard, He, Liyan, Turner, Douglas H.·1992·Nucleic Acids Research

Abstract

Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.

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  • 2'-5' linked RNA duplexes have a much lower Tm than natural RNA duplexes.

    “It has been known for many years that 2 ' -5 ' linked RNA duplexes have a much lower Tm than natural RNA duplexes [23].”

    From The eightfold path to non-enzymatic RNA replication (2012) · cites this paper as [23]

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Research works printed but not linked: 6

  1. Zamecnik, P. C. and Stephenson, M. L. (1978) Proc. Natl. Acad. Sci. U.S.A., 75, 280-284.
  2. Usher, D. A. and McHale, A. H. (1976) Proc. Natl. Acad. Sci. U.S.A., 73, 1149-1153.
  3. Parthasarathy, R., Malik, M. and Fridey, S. M. (1982) Proc. Natl. Acad. Sci. U.S.A., 79, 7292-7296.
  4. Ogilvie, K. K., Usman, N., Nicognosian, K. and Cedegren, R. J. (1989) Proc. Natl. Acad. Sci. U.S.A., 85, 5764-5768.
  5. Richards, E. G. (1975) in Handbook of Biochemistry and Molecular Biology: Nucleic Acids (Fasmak, G. D. Ed.) 3rd Vol. I, p. 597, CRC Press Cleveland, OH.
  6. Freier, S. M., Kierzek, R., Jaeger, J. A., Sugimoto, N., Caruthers, M. H., Neilson, T. and Turner, D. H. (1986) Proc. Natl. Acad. Sci. U.S.A., 83, 9373-9377.

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Authors
Kierzek, R., He, L., & Turner, D. H.
Affiliations
  • Institute of Bioorganic Chemistry, Polish Academy of Sciences (PL)
  • University of Rochester (US)
Corresponding author
Turner, Douglas H.
Venue
Nucleic Acids Research
Published
1992
Volume
20
Issue
7
Pages
1685-1690
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2034295729
Cited by
79
References
31
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2020: 2 · 2017: 1 · 2016: 2 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
1.99
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (GM 22939)
  • National Institute of General Medical Sciences (GM 22939, R01GM022939)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords
duplex stability, UV melting, circular dichroism, imino proton NMR, NOE spectroscopy, RNA evolution, RNA secondary structure
MeSH terms
Base Sequence, Circular Dichroism, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Oligoribonucleotides, Spectrophotometry, Ultraviolet, Temperature

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