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Covalent attachment of synthetic DNA to self-assembled monolayer films
Open accessAbstract
The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.
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Citations
Cited by
Array-Based Electrical Detection of DNA with Nanoparticle Probes
Park, S.-J., Taton, T. A., & Mirkin, C. A. (2002) · Science · doi:10.1126/science.1067003 · Cited by 1,515
What Controls the Melting Properties of DNA-Linked Gold Nanoparticle Assemblies?
Jin, R., Wu, G., Li, Z., Mirkin, C. A., & Schatz, G. C. (2003) · Journal of the American Chemical Society · doi:10.1021/ja021096v · Cited by 1,359
Electrochemistry of Nucleic Acids
Paleček, E., & Bartošík, M. (2012) · Chemical Reviews · doi:10.1021/cr200303p · Cited by 668
Dynamic force spectroscopy of single DNA molecules
Strunz, T., Oroszlan, K., Schäfer, R., & Güntherodt, H.-J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.20.11277 · Cited by 580
Microarray fabrication with covalent attachment of DNA using Bubble Jet technology
Okamoto, T., Suzuki, T., & Yamamoto, N. (2000) · Nature Biotechnology · doi:10.1038/74507 · Cited by 440
Metadata
- Authors
- Chrisey, L.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-08-01
- Volume
- 24
- Issue
- 15
- Pages
- 3031-3039
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2132993311
- Cited by
- 328
- References
- 0
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
