The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Dynamic force spectroscopy of single DNA molecules
Open accessAbstract
To explore the analytic relevance of unbinding force measurements between complementary DNA strands with an atomic force microscope, we measured the forces to mechanically separate a single DNA duplex under physiological conditions by pulling at the opposite 5′-ends as a function of the loading rate (dynamic force spectroscopy). We investigated DNA duplexes with 10, 20, and 30 base pairs with loading rates in the range of 16–4,000 pN/s. Depending on the loading rate and sequence length, the unbinding forces of single duplexes varied from 20 to 50 pN. These unbinding forces are found to scale with the logarithm of the loading rate, which is interpreted in terms of a single energy barrier along the mechanical separation path. The parameters describing the energy landscape, i.e., the distance of the energy barrier to the minimum energy along the separation path and the logarithm of the thermal dissociation rate, are found to be proportional to the number of base pairs of the DNA duplex. These single molecule results allow a quantitative comparison with data from thermodynamic ensemble measurements and a discussion of the analytic applications of unbinding force measurements for DNA.
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Cites
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Energy landscapes of receptor–ligand bonds explored with dynamic force spectroscopy
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Mechanical and chemical unfolding of a single protein: A comparison
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Cited by
Ribas-Arino, J., & Marx, D. (2012) · Chemical Reviews · doi:10.1021/cr200399q · Cited by 448
AFM: a versatile tool in biophysics
Alessandrini, A., & Facci, P. (2005) · Measurement Science and Technology · doi:10.1088/0957-0233/16/6/r01 · Cited by 384
Origin of Friction Derived from Rupture Dynamics
Suda, H. (2001) · Langmuir · doi:10.1021/la0106384 · Cited by 25
Metadata
- Authors
- Strunz, T., Oroszlan, K., Schäfer, R., & Güntherodt, H.-J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-09-28
- Volume
- 96
- Issue
- 20
- Pages
- 11277-11282
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2108242211
- Cited by
- 580
- References
- 26
- FWCI
- 14.37
- Type
- journal article
- Language
- en
- Open access
- green
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- Strunz, T., Oroszlan, K., Schäfer, R., & Güntherodt, H.-J. (1999). Dynamic force spectroscopy of single DNA molecules. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.96.20.11277
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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