The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Antisense Properties of Duplex- and Triplex-Forming PNAs
Open accessAbstract
The potential of peptide nucleic acids (PNAs) as specific inhibitors of translation has been studied. PNAs with a mixed purine/pyrimidine sequence form duplexes, while homopyrimidine PNAs form (PNA)2/RNA triplexes with complementary sequences on RNA. We show here that neither of these PNA/RNA structures are substrates for RNase H. Translation experiments in cell-free extracts showed that a 15mer duplex-forming PNA blocked translation in a dose-dependent manner when the target was 5'-proximal to the AUG start codon on the RNA, whereas similar 10-, 15- or 20mer PNAs had no effect when targeted towards sequences in the coding region. Triplex-forming 10mer PNAs were efficient and specific antisense agents with a target overlapping the AUG start codon and caused arrest of ribosome elongation with a target positioned in the coding region of the mRNA. Furthermore, translation could be blocked with a 6mer bisPNA or with a clamp PNA, forming partly a triplex, partly a duplex, with its target sequence in the coding region of the mRNA.
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Citations
Cites
Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide
Nielsen, P. E., Egholm, M., Berg, R. H., & Buchardt, O. (1991) · Science · doi:10.1126/science.1962210 · Cited by 3,332
Self-citationPNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules
Egholm, M., Buchardt, O., Christensen, L., Behrens, C., Freier, S. M., Driver, D. A., Berg, R. H., Kim, S. K., Norden, B., & Nielsen, P. E. (1993) · Nature · doi:10.1038/365566a0 · Cited by 2,079
Self-citation
Cited by
Drug Transport across the Blood–Brain Barrier
Pardridge, W. M. (2012) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2012.126 · Cited by 1,994
Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future
Ray, A., & Nordén, B. (2000) · The FASEB Journal · doi:10.1096/fasebj.14.9.1041 · Cited by 416
Peptide Nucleic Acid. A Molecule with Two Identities
Nielsen, P. E. (1999) · Accounts of Chemical Research · doi:10.1021/ar980010t · Cited by 380
Self-citationA Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity
Tse, W. C., & Boger, D. L. (2004) · Accounts of Chemical Research · doi:10.1021/ar030113y · Cited by 288
Triple helix formation and the antigene strategy for sequence-specific control of gene expression
Praseuth, D., Guieysse, A. L., & Hélène, C. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00149-9 · Cited by 262
Metadata
- Authors
- Knudsen, H., & Nielsen, P. E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-02-01
- Volume
- 24
- Issue
- 3
- Pages
- 494-500
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2010476099
- Cited by
- 256
- References
- 2
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Knudsen, H., & Nielsen, P. E. (1996). Antisense Properties of Duplex- and Triplex-Forming PNAs. Nucleic Acids Research. https://doi.org/10.1093/nar/24.3.494
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