Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide
Abstract
A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.
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PNA can form a double strand with itself and with DNA or RNA.
“PNA can form a double strand with itself and with DNA or RNA, which supports the idea that it could have acted as a replication molecule (Nielsen et al. 1991).”
From The Emergence of Life (2019) · cites this paper as Nielsen et al. 1991
PNA could have acted as a replication molecule.
“PNA can form a double strand with itself and with DNA or RNA, which supports the idea that it could have acted as a replication molecule (Nielsen et al. 1991).”
From The Emergence of Life (2019) · cites this paper as Nielsen et al. 1991
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- Authors
- Nielsen, P. E., Egholm, M., Berg, R. H., & Buchardt, O.
- Affiliations
- University of Copenhagen (DK)
- Polymer Group, Materials Department, Risø National Laboratory, DK-4000 Roskilde, Denmark.
- ORCID
- Nielsen, Peter E.
- Venue
- Science
- Published
- 1991-12-06
- Volume
- 254
- Issue
- 5037
- Pages
- 1497-1500
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2019818589
- Cited by
- 3,332
- References
- 0
- Citations by year
- 2026: 62 · 2025: 78 · 2024: 77 · 2023: 91 · 2022: 95 · 2021: 110 · 2020: 127 · 2019: 101 · 2018: 101 · 2017: 102 · 2016: 103 · 2015: 106 · 2014: 115 · 2013: 115 · 2012: 107
- FWCI
- 12.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- strand displacement, DNA recognition, PNA-DNA hybrids, sequence-specific hybridization, oligonucleotide design
- MeSH terms
- Base Sequence, Molecular Sequence Data, Nylons, Oligonucleotides, Photochemistry, Thermodynamics, Molecular Structure
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