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Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA
Open accessAbstract
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon generated by NASBA, producing a specific fluorescent signal that can be monitored in real-time. The assay is rapid, sensitive and specific. As RNA amplification and detection can be carried out in unopened vessels, it minimizes the risk of carry-over contaminations. Robustness has been verified on real-world samples. This homogeneous assay, called AmpliDet RNA, is a significant improvement over current detection methods for NASBA amplicons and is suitable for one-tube applications ranging from high-throughput diagnostics to in vivo studies of biological activities.
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Cited by
Lanciotti, R. S., & Kerst, A. J. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.12.4506-4513.2001 · Cited by 267
Fox, J. D., Han, S., Samuelson, A., Zhang, Y., Neale, M. L., & Westmoreland, D. (2002) · Journal of Clinical Virology · doi:10.1016/s1386-6532(01)00241-4 · Cited by 84
Changing approaches to the diagnosis of tuberculosis
SCHLUGER, N. W. (2001) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.164.11.2008100 · Cited by 60
Metadata
- Authors
- Leone, G.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-05-01
- Volume
- 26
- Issue
- 9
- Pages
- 2150-2155
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2141627564
- Cited by
- 306
- References
- 0
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Leone, G. (1998). Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA. Nucleic Acids Research. https://doi.org/10.1093/nar/26.9.2150
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