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Versatile derivatisation of solid support media for covalent bonding on DNA-microchips
Open accessAbstract
A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces. Bonding was found to be stable even after some 30 cycles of stripping. Due to a dendrimeric structure of the linker molecule, the loading can be modified in a controlled manner and increased beyond the capacity of glass without negative effects on hybridisation efficiency. Also, the chemistry warrants the modulation of other surface properties such as charge or hydrophobicity. Preferentially, attachment of nucleic acids takes place only via the terminal amino-group of amino-modified oligonucleotides or the terminal hydroxyl-group of unmodified molecules so that the entire molecule is accessible to probe hybridisation. This derivatisation represents a support chemistry versatile enough to serve nearly all current forms of DNA-arrays or microchips.
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Cited by
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Queffélec, C., Petit, M., Janvier, P., Knight, D. A., & Bujoli, B. (2012) · Chemical Reviews · doi:10.1021/cr2004212 · Cited by 868
Sequence-Specific Label-Free DNA Sensors Based on Silicon Nanowires
Li, Z., Chen, Y., Li, X., Kamins, T. I., Nauka, K., & Williams, R. S. (2004) · Nano Letters · doi:10.1021/nl034958e · Cited by 706
Celis, J. E., Kruhøffer, M., Gromova, I., Frederiksen, C., Østergaard, M., Thykjaer, T., Gromov, P., Yu, J., Pálsdóttir, H., Magnusson, N., et al. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01771-3 · Cited by 314
Solid supports for microarray immunoassays
Kusnezow, W., & Hoheisel, J. D. (2003) · Journal of Molecular Recognition · doi:10.1002/jmr.625 · Cited by 277
Self-citationGenome-wide Analysis of the Response to Cell Wall Mutations in the Yeast Saccharomyces cerevisiae
Lagorce, A., Hauser, N. C., Labourdette, D., Rodriguez, C., Martin-Yken, H., Arroyo, J., Hoheisel, J. D., & François, J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211604200 · Cited by 254
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Metadata
- Authors
- Beier, M., & Hoheisel, J. D.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-05-01
- Volume
- 27
- Issue
- 9
- Pages
- 1970-1977
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2109056556
- Cited by
- 274
- References
- 0
- FWCI
- 4.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Beier, M., & Hoheisel, J. D. (1999). Versatile derivatisation of solid support media for covalent bonding on DNA-microchips. Nucleic Acids Research. https://doi.org/10.1093/nar/27.9.1970
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