The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes
Open accessAbstract
The uptake and expression of extracellular DNA has been established as a mechanism for horizontal transfer of genes between bacterial species. Such transfer can support acquisition of advantageous elements, including determinants that affect the interactions between infectious organisms and their hosts. Here we show that erythrocyte-stage Plasmodium falciparum malaria parasites spontaneously take up DNA from the host cell cytoplasm into their nuclei. We have exploited this finding to produce levels of reporter expression in P.falciparum that are substantially improved over those obtained by electroporation protocols currently used to transfect malaria parasites. Parasites were transformed to a drug-resistant state when placed into cell culture with erythrocytes containing a plasmid encoding the human dihydrofolate reductase sequence. The findings reported here suggest that the malaria genome may be continually exposed to exogenous DNA from residual nuclear material in host erythrocytes.
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Cites
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Cited by
Janse, C. J., Ramesar, J., & Waters, A. P. (2006) · Nature Protocols · doi:10.1038/nprot.2006.53 · Cited by 631
Tonkin, C. J., van Dooren, G. G., Spurck, T. P., Struck, N. S., Good, R. T., Handman, E., Cowman, A. F., & McFadden, G. I. (2004) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2004.05.009 · Cited by 465
High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
Janse, C. J., Franke-Fayard, B., Mair, G. R., Ramesar, J., Thiel, C., Engelmann, S., Matuschewski, K., Gemert, G. J. V., Sauerwein, R. W., & Waters, A. P. (2006) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2005.09.007 · Cited by 437
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Metadata
- Authors
- Deitsch, K. W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-02-01
- Volume
- 29
- Issue
- 3
- Pages
- 850-853
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2086438483
- Cited by
- 318
- References
- 21
- FWCI
- 9.23
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
